| 冯佳,田芮萌,芦桂林,秦文娟.心肌超声造影心动图联合斑点追踪成像评价2-氨基乙氧基二苯基硼酸酯对砷中毒大鼠心脏保护作用[J].中国医学影像技术,2024,40(1):7~13 |
| 心肌超声造影心动图联合斑点追踪成像评价2-氨基乙氧基二苯基硼酸酯对砷中毒大鼠心脏保护作用 |
| Myocardial contrast echocardiography combined with speckle tracking imaging for evaluating protective effect of 2-aminoethoxydiphenyl borate on heart of arsenic poisoned rats |
| 投稿时间：2023-11-08 修订日期：2023-12-27 |
| DOI：10.13929/j.issn.1003-3289.2024.01.002 |
| 中文关键词: 大鼠 心脏 砷中毒 2-氨基乙氧基二苯基硼酸酯 超声心动描记术 |
| 英文关键词:rats heart arsenic poisoning 2-aminoethoxydiphenyl borate echocardiography |
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| 中文摘要: |
| 目的 观察心肌超声造影心动图（MCE）联合斑点追踪成像（STI）评价2-氨基乙氧基二苯基硼酸酯（2-APB）对砷中毒大鼠心脏保护作用的价值。方法 将40只大鼠随机分为三氧化二砷（ATO）中毒后低剂量2-APB干预组（2-APBL组）、ATO中毒后高剂量2-APB干预组（2-APBH组）、单纯ATO中毒组（ATO组）、单纯2-APB干预组（2-APB组）及对照组，每组8只。对2-APBL组、2-APBH组及ATO组均经腹腔注射ATO 5 mg/kg体质量，对2-APB组和对照组注射等量生理盐水；之后分别以2、4、4 mg/kg体质量对2-APBL组、2-APBH组和2-APB组注射2-APB，对ATO组和对照组注射等量生理盐水；行MCE及STI检查，检测血清心肌损伤标志物及心肌肌浆网Ca2+-ATP酶（SERCA）；最后行心肌病理学检查。观察组间各参数差异，并行相关性分析。结果 5组左心室短轴缩短率（LVFS）、左心室射血分数（LVEF）、曲线上升斜率（WIS）、峰值信号强度（PI）、WIS×PI、曲线下面积（AUC）、各层心肌整体圆周应变（GCS）及谷草转氨酶（GOT）、肌酸激酶（CK）、乳酸脱氢酶（LDH）、SERCA差异均有统计学意义（P均<0.05），且基本依ATO组、2-APBL组、2-APBH组、2-APB组/对照组次序递增或递减（P均<0.05）。WIS、PI、WIS×PI、心内膜下心肌GCS、中层心肌GCS、心外膜下心肌GCS均与SERCA呈正相关（r=0.874、0.893、0.920、0.916、0.848、0.783，P均<0.05）。结论 MCE联合STI、尤其WIS×PI和心内膜下心肌GCS可用于评估2-APB对砷中毒大鼠心脏的保护作用。 |
| 英文摘要: |
| Objective To observe the value of myocardial contrast echocardiography (MCE) combined with speckle tracking imaging (STI) for evaluating the protective effect of 2-aminoethoxydiphenyl borate (2-APB) on heart of arsenic poisoned rats. Methods Forty rats were randomly divided into low- and high-dose 2-APB intervention after arsenic trioxide (ATO) poisoned group (2-APBL group and 2-APBH group), simple ATO poisoned group (ATO group), simple 2-APB intervention group (2-APB group) and control group (each n=8). 5 mg/kg ATO were intraperitoneally injected in 2-APBL group, 2-APBH group and ATO group, while equal dose physiological saline were injected in 2-APB group and control group, respectively. Then 2, 4 and 4 mg/kg 2-APB were intraperitoneally injected in 2-APBL group, 2-APBH group and 2-APB group, while equal doses physiological saline were injected in ATO group and control group, respectively. MCE and STI were performed, serum myocardial injury markers were tested, sarcoplasmic reticulum Ca2+-ATPase (SERCA) was examinated, and finally myocardial pathological examination was performed. The above indexes were compared among groups and between each 2 groups, and correlation analysis was performed. Results Significant differences of left ventricular fractional shortening (LVFS), left ventricular ejection fraction (LVEF), wash in slope (WIS), peak intensity (PI), WIS×PI, area under the curve (AUC), global circumferential strain (GCS) in each layer of myocardium, as well as glutamic-oxaloacetic transaminase (GOT), creatine kinase (CK), lactate dehydrogenase (LDH) and SERCA were found among 5 groups (all P<0.05). The above indexes substantially increased or decreased sequentially ATO group, 2-APBL group, 2-APBH group and 2-APB group/control group (all P<0.05). WIS, PI, WIS×PI and GCS of rat endo-myocardium, GSC of mid-myocardium and GCS of epi-myocardium all positively correlated with SERCA (r=0.874, 0.893, 0.920, 0.916, 0.848, 0.783, all P<0.05). Conclusion MCE combined with STI, especially WIS×PI and GCS of endo-myocardium could be used to evaluate the protective effect of 2-APB on heart of arsenic poisoned rats. |
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