黄通,王志刚,杜之渝.载单宁酸铁及二氢卟吩纳米粒用于光声成像及杀伤视网膜母细胞瘤Y79细胞[J].中国医学影像技术,2022,38(7):968~974
载单宁酸铁及二氢卟吩纳米粒用于光声成像及杀伤视网膜母细胞瘤Y79细胞
Fe-tannic acid and chlorin e6 loaded nanoparticle for photoacoustic imaging and killing retinoblastoma Y79 cells
投稿时间:2021-12-14  修订日期:2022-04-21
DOI:10.13929/j.issn.1003-3289.2022.07.002
中文关键词:  纳米粒子  视网膜母细胞瘤  光声成像
英文关键词:nanoparticles  retinoblastoma  photoacoustic imaging
基金项目:重庆市基础研究与前言探索项目(cstc2018jcyjAX0562)。
作者单位E-mail
黄通 重庆医科大学附属第二医院眼科, 重庆 400010
重庆医科大学生物医学工程学院超声医学工程重点实验室, 重庆 400010 
 
王志刚 超声分子影像学重庆市医学重点实验室, 重庆 400010  
杜之渝 重庆医科大学附属第二医院眼科, 重庆 400010 dr.duzhiyu@163.com 
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中文摘要:
       目的 制备载单宁酸铁(Fe TA)及二氢卟吩(Ce6)纳米粒,观察其用于光声(PA)成像及杀伤视网膜母细胞瘤(RB)Y79细胞的价值。方法 制备Fe TA/PLGA/Ce6纳米粒并检测其特性,观察其体内、体外PA成像能力及对人源性RB Y79细胞的杀伤能力。结果 成功制备的Fe TA/PLGA/Ce6纳米粒基本特性良好,光热稳定性优异,用于实时PA成像效果较好。纳米粒浓度越高,经808 nm激光辐照后样品升温幅度越大。各种浓度纳米粒对人视网膜色素上皮细胞均无明显细胞毒性。于Y79细胞中加入纳米粒后,荧光强度随时间延长而增高;激光组(Y79细胞+激光辐照)、Fe TA/PLGA/Ce6组(Y79细胞+纳米粒)、Fe TA/PLGA/Ce6+激光组(Y79细胞+纳米粒+激光辐照)及空白对照组(Y79细胞)于Y79细胞内的活性氧产量百分比分别为28.01%、55.41%、42.74%及26.57%。Fe TA/PLGA/Ce6纳米粒及激光辐照共同作用可使Y79细胞大量凋亡。结论 成功制备的Fe TA/PLGA/Ce6纳米粒用于PA成像效果较好;用于光热治疗联合化学动力疗法可有效杀伤体外Y79细胞。
英文摘要:
      Objective To observe the value of Fe-tannic acid (Fe TA) and chlorin e6 (Ce6) loaded nanoparticle for photoacoustic (PA) imaging and killing retinoblastoma (RB) Y79 cells. Methods Fe TA/PLGA/Ce6 nanoparticles were prepared and the properties were tested, and the value for in vivo and in vitro PA imaging, also for killing human RB Y79 cells were observed. Results Fe TA/PLGA/Ce6 nanoparticles were successfully prepared, which had good basic properties, excellent thermo-optical stability and real-time PA imaging ability. The higher the concentration of Fe TA/PLGA/Ce6 nanoparticles, the higher the temperature of sample irradiated by 808 nm laser. No obvious cytotoxicity to human retinal pigment epithelial cells was detected in Fe TA/PLGA/Ce6 nanoparticles with different concentrations. After Fe TA/PLGA/Ce6 nanoparticles were added to Y79 cells, the fluorescence intensity increased with time. The percentage of reactive oxygen species production in Y79 cells of laser group (Y79 cells+laser irradiation), Fe TA/PLGA/Ce6 group (Y79 cells+Fe TA/PLGA/Ce6 nanoparticles), Fe TA/PLGA/Ce6+laser group (Y79 cells+Fe TA/PLGA/Ce6 nanoparticles+laser irradiation) and blank control group (Y79 cells) was 28.01%, 55.41%, 42.74% and 26.57%, respectively. Fe TA/PLGA/Ce6 nanoparticles and laser irradiation could induce a large number of apoptosis of Y79 cells. Conclusion The successfully prepared Fe TA/PLGA/Ce6 nanoparticles had good PA imaging ability, which could effectively kill Y79 cells in vitro through combination of photothermal therapy and chemodynamic therapy.
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