| 熊婧彤,伍建林,张清,沈晶.扩散峰度成像动态定量检测兔轻度脑外伤模型早期变化及其与β-淀粉样前体蛋白阳性表达的相关性[J].中国医学影像技术,2018,34(9):1312~1317 |
| 扩散峰度成像动态定量检测兔轻度脑外伤模型早期变化及其与β-淀粉样前体蛋白阳性表达的相关性 |
| Dynamic and quantitative diffusion kurtosis imaging of rabbit mild traumatic brain injury models in early phase and the relationship with positive expression of β-amyloid precursor protein |
| 投稿时间：2017-08-20 修订日期：2018-03-12 |
| DOI：10.13929/j.1003-3289.201708089 |
| 中文关键词: 轻度脑外伤 动物模型 扩散峰度成像 β-淀粉样前体蛋白 |
| 英文关键词:Mild traumatic brain injury Animal model Diffusion kurtosis imaging β-amyloid precursor protein |
| 基金项目:国家自然科学基金面上项目（30870699）。 |
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| 中文摘要: |
| 目的 观察兔轻度脑外伤（mTBI）模型损伤早期扩散峰度成像（DKI）测量值动态变化，分析其与β-淀粉样前体蛋白（β-APP）阳性表达的相关性。方法 将15只兔随机分为损伤组（12只，按建模后损伤时间分为伤后6 h、24 h、48 h、72 h亚组，每亚组3只）及对照组（建模前，3只），进行MR检查并测量平均扩散峰度（MK）、轴向扩散峰度（K//）与径向扩散峰度（K┴）值。之后处死动物，取脑行HE、β-APP染色观察。采用多个独立样本秩和检验比较DKI指标在建模前后各时间点间的总体变化，以两独立样本秩和检验比较建模前与建模后各时间点的差异；采用单因素方差分析比较β-APP染色阳性轴突数在各时间点之间的变化，以Spearman相关分析观察其与DKI测量值的相关性。结果 各测量脑区MK、K//及K┴值随时间变化趋势不同。建模后β-APP阳性轴突数总体呈增加后减少又增加趋势。皮层下白质、中脑、桥脑及延髓MK值与β-APP阳性轴突数呈正相关，脑干区K//及K┴值多与β-APP阳性轴突数呈正相关。结论 DKI技术可早期定量评估mTBI脑组织微结构损伤；DKI测量值多与β-APP阳性表达呈正相关。 |
| 英文摘要: |
| Objective To explore the value of diffusion kurtosis imaging (DKI) in rabbit models of mild traumatic brain injury (mTBI) in early phase, and to observe the relationship with positive expression of β-amyloid precursor protein (β-APP). Methods Totally 15 rabbits were randomly divided into injured group (according to injury duration time divided into 6 h, 24 h, 48 h, 72 h subgroups, each n=3) and control group (n=3). MR scanning was performed. And the mean kurtosis (MK), axial kurtosis (K//) and radial kurtosis(K┴) values were measured. Then the rabbits were scarified, HE and β-APP staining of the brains were performed. The general variations of DKI values in measured brain areas were analyzed among different timepoints before and after model establishment with Kruskal-Wallis test, and the paired comparisons were performed with two independent samples rank sum test. One-way ANOVA was applied in comparison to the number of β-APP positive axons by time, and the correlation with DKI measured values were analyzed with Spearman correlation analysis. Results Trends of MK, K// and K┴ values in each brain areas were different. The numbers of β-APP positive axons mainly manifested as the trends of increasing before decreasing, and then rising again. MK values in subcortical white matter, pons and medulla were positively correlated with number of β-APP positive axons. K// and K┴ values in almost all measured brainstem areas showed positive relationship with the number of β-APP positive axons. Conclusion DKI was effective in quantitatively assessing micro-structure injuries in mTBI rabbit models in the early phase. Most of DKI values showed positive relationship with positive expression of β-APP. |
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