| 杨双风,王岩,唐晓璐,王伟,张阔,吴荣昌,韩鸿宾,彭芸.应用磁共振示踪技术分析幼年及成年大鼠丘脑细胞外间隙扩散特性[J].中国医学影像技术,2016,32(9):1315~1319 |
| 应用磁共振示踪技术分析幼年及成年大鼠丘脑细胞外间隙扩散特性 |
| Diffusion features comparision of thalamus extracellular space in infant and adult rat with MRI |
| 投稿时间：2016-01-26 修订日期：2016-06-27 |
| DOI：10.13929/j.1003-3289.2016.09.001 |
| 中文关键词: 细胞外间隙 钆喷酸葡胺 扩散磁共振成像 |
| 英文关键词:Extracellular space Gadolinium-diethylene triaminepentaacetic acid Diffusion magnetic resonance imaging |
| 基金项目:国家自然科学基金（31271161、81471633）。 |
| 作者 | 单位 | E-mail | | 杨双风 | 首都医科大学附属北京儿童医院影像中心, 北京 100045 | | | 王岩 | 首都医科大学附属北京儿童医院影像中心, 北京 100045 | | | 唐晓璐 | 首都医科大学附属北京儿童医院影像中心, 北京 100045 | | | 王伟 | 北京大学第三医院放射科, 北京 100191
北京市磁共振成像设备与技术重点实验室, 北京 100191 | | | 张阔 | 北京大学医学部实验动物科学部, 北京 100191 | | | 吴荣昌 | 首都医科大学附属北京儿童医院影像中心, 北京 100045 | | | 韩鸿宾 | 北京大学第三医院放射科, 北京 100191
北京市磁共振成像设备与技术重点实验室, 北京 100191 | hanhongbin@bjmu.edu.cn | | 彭芸 | 首都医科大学附属北京儿童医院影像中心, 北京 100045 | ppengyun@hotmail.com |
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| 中文摘要: |
| 目的 采用磁共振示踪法定量比较10日龄及成年SD大鼠丘脑细胞外间隙扩散性能。方法 将浓度为 10mmol/L示踪剂Gd-DTPA导入大鼠丘脑区脑细胞外间隙内，分别于给药前和给药后按一定时间间隔连续采集脑MR图像。在图像后处理软件上进行配准、减影等处理，将信号强度增量换算为Gd-DTPA的浓度值，利用经典扩散方程计算出脑细胞外间隙内的微观参数，包括有效扩散系数（D*）、纡曲度（λ）、清除速率常数（k'）和宏观参数示踪剂分布容积分数（Vd%）。结果 10日龄鼠丘脑的D*低于成年鼠，λ、k'、Vd%均高于成年鼠（P均<0.05）。结论 10日龄及成年大鼠脑细胞外间隙的扩散参数存在显著差异，可以为发育相关脑病的诊治及机制探索提供依据。 |
| 英文摘要: |
| Objective To compare diffusion features of thalamus extracellular space of the postnatal 10 days (P10) and adult rats with MRI tracer method. Methods MR scanning was performed before and after the intracranial injection of Gd-DTPA (10 mmol/L) into the extracellular space (ECS) of thalamus as a tracer. A Matlab-based software was developed to co-register and subject the MR images of the same rat before and after injection. According to the modified diffusion equation and the linear relationship between the signal intensity and the concentration of Gd-DTPA, the diffusion parameters were obtained, including effective diffusion coefficient (D*), clearance rate (k'), tortuosity(λ) and the volume fraction of distribution (Vd%). Results D* in the thalamus of P10 rats was significantly less than that of the adult rats, and λ of P10 rats was significantly greater than that of the adult rats; k' and Vd% in the thalamus of P10 rats were significantly greater than those of the adult rats (all P<0.05). Conclusion There are significant differences in the diffusion parameters between P10 and adult rat thalami, which provides a reference for the diagnosis and treatment of development related brain diseases. |
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