杜联芳,张青萍,刘望彭.诊断超声对不同发育阶段大鼠睾丸组织凋亡的研究[J].中国医学影像技术,2000,16(7):0609~610 |
诊断超声对不同发育阶段大鼠睾丸组织凋亡的研究 |
Study of Apoptosis in Different Age Rat Testes af ter Diagnostic Ultrasound Radiation |
投稿时间:1999-12-12 |
DOI: |
中文关键词: 睾丸 超声 凋亡 |
英文关键词:Testes Ultrasound Apoptosis |
基金项目: |
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中文摘要: |
目的 探讨诊断超声对不同发育阶段大鼠睾丸组织生精细胞凋亡的影响。方法 36 只SD 雄性大鼠随
机分为6 组。应用HP8500 彩超仪对大鼠睾丸进行30 min 持续照射,24h 后取睾丸进行凋亡检测。结果 不同年龄
组生精细胞均有不同程度的细胞凋亡,以出生后30d 凋亡细胞数最多,随年龄增长凋亡细胞数有减少趋势。超声照
射30min 后各实验组凋亡细胞数均有显著增加。结论 不同发育阶段大鼠睾丸生精细胞凋亡率不同,超声照射
30min 后可引起各组生 |
英文摘要: |
Objective To investigate the germ cell apoptosis in different age rat testes after diagnostic ultrasound. Methods 36 male Sprague-Danles rats were randomly separated into six groups of six according to different ages. Rat testes
were radiated by HP 8500 color Doppler sonography with 7. 5MHz linear phased array transducer for 30 minutes. Their
testes were removed at 24 hour after radiation. Apoptosis was detected with TUNEL (in situterminal deoxynucleotityl
transferase-mediated dUTP nick end labeling) . Results The incidence of testicular germ cell apoptosis in all groups was
detected and peaked at post-birth 30 days ; after radiation apoptotic incidence in all experimental groups was significantly
higher than control groups ( P < 01001) . Conclusion Germ cell apoptotic incidence varied in different age rat ; radiation
continuously 30 minutes by diagnostic ultrasound may result in germ cell apoptosis increasing in the different age rat testes. |
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