| 周丽,魏世杰.超声生物显微镜评估大鼠急、慢性心肌肥厚模型左心室心肌形态及功能的可行性[J].中国医学影像技术,2020,36(3): |
| 超声生物显微镜评估大鼠急、慢性心肌肥厚模型左心室心肌形态及功能的可行性 |
| The feasibility 0f assessing left ventricular myocardial morphology and function in rat models with acute and chronic myocardial hypertrophy by ultrasound biomicroscopy |
| 投稿时间：2019-04-16 修订日期：2020-03-16 |
| DOI： |
| 中文关键词: 心肌病, 肥厚性 显微镜检查, 声学 超声检查 异丙肾上腺素 疾病模型, 动物 |
| 英文关键词:cardiomyopathy, hypertrophic microscopy, acoustic ultrasonography isoproterenol disease models,animal |
| 基金项目:结肠靶向型5-FU-Succinyl-β-CD共价物的构建及其非临床PK/PD的研究 |
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| 中文摘要: |
| [摘 要] 目的 探讨超声生物显微镜（UBM）测量异丙肾上腺素（ISO）所致大鼠急、慢性心肌肥厚模型左心室形态及功能参数的可行性。方法 将40只SD大鼠随机均分为4组。急性模型组大鼠皮下注射ISO 85 mg/kg体质量，1次/天，连续给药2天；慢性模型组皮下注射ISO 5 mg/kg体质量，1次/天，连续给药7天；对照组均给予等量、等时间生理盐水皮下注射。采用UBM测量以下参数：左心室前壁厚度（LVAWD）、左心室后壁厚度（LVPWD）、左心室舒张末期内径（LVEDD）、左心室收缩末期内径（LVESD）、左心室舒张末期容积（LVEDV）、左心室收缩末期容积（LVESV）、左心室射血分数（LVEF）、左心室短轴缩短率（LVFS）及左心室质量。处死大鼠后对左心室质量进行精密称重。结果 急性模型组较急性对照组LVAWD、LVPWD增加（P均＜0.05）；慢性模型组较慢性对照组LVAWD、LVPWD、LVESD及LVESV增大，LVEF、LVFS降低（P均＜0.05）；慢性模型组较急性肥厚模型组LVEDD、LVESD、LVEDV、LVESV增大，LVEF、LVFS降低（P均＜0.05）。4组UBM所测左心室心肌质量均高于精确称重（P均＜0.05），两种方法所测急性模型组左心室质量均低于慢性模型组（P均＜0.05）。4组UBM所测左心室质量与精确称重所测左心室心肌质量均呈正相关（P均＜0.05）。结论 UBM能够无创评估ISO所致大鼠急、慢性心肌肥厚模型左心室形态及功能。
[关键词] 心肌病, 肥厚性；显微镜检查, 声学；超声检查；异丙肾上腺素；疾病模型, 动物 |
| 英文摘要: |
| [Abstract] Objective To investigate the feasibility of measuring morphological and functional parameters of left ventricle in rat models with acute and chronic myocardial hypertrophy induced by isoproterenol (ISO) using ultrasound biomicroscopy (UBM). Methods 40 SD rats were randomly divided into 4 groups. Rats in the acute myocardial hypertrophy model group were given subcutaneous injection with ISO 85 mg·kg-1 once daily for 2 days. The ones in chronic myocardial hypertrophy model group were given subcutaneous injection with ISO 5 mg·kg-1 once daily for 7 days. The control groups were given equal amount saline with Subcutaneous injection for equal time.The following parameters were measured by UBM: left ventricular anterior wall diameter (LVAWD), left ventricular posterior wall diameter (LVPWD), left ventricular end diastolic diameter (LVEDD), left ventricular end systolic diameter (LVESD), left ventricular end diastolic volume (LVEDV),Left ventricular end systolic volume (LVESV), left ventricular ejection fraction (LVEF), left ventricular fractional shortning (LVFS) and left ventricular myocardial mass. After the rats were sacrificed,the left ventricular myocardial mass was weighed accurately. Results Compared with the acute control group, LVAWD and LVPWD increased in the acute model group (all P <0.05).Compared with the chronic control group, LVAWD, LVPWD, LVESD and LVESV increased in the chronic model group, while LVEF and LVFS decreased (all p<0.05).Compared with the acute model group, LVEDD, LVESD, LVEDV and LVESV of the chronic model group increased, but LVEF and LVFS decreased (all P <0.05). Left ventricular myocardial mass measured in the 4 groups by UBM was higher than anatomical accurate weighing (all P <0.05). Left ventricular myocardial mass of the acute model group measured by 2 methods was lower than that of the chronic model group (all P <0.05). The left ventricular myocardial mass measured by UBM in 4 groups was positively correlated with the value measured by anatomical accurate weighing (all P <0.05). Conclusion UBM can evaluate left ventricular morphology and function non-invasively in rats with acute and chronic myocardial hypertrophy induced by ISO.
[Keywords] cardiomyopathy, hypertrophic; microscopy, acoustic; ultrasonography; isoproterenol; disease models,animal |
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