| 赵丽娜,黄丹,李晓怡,赵悦瑶,杨国柠,李莎,谢瑾,张蓓.基于不同帧频显微超声造影(SR CEUS)评估4T1小鼠乳腺癌微循环[J].中国医学影像技术,2026,42(6):821~824 |
| 基于不同帧频显微超声造影(SR CEUS)评估4T1小鼠乳腺癌微循环 |
| Different frame frequency super resolution contrast-enhanced ultrasound (SR CEUS) for evaluating microcirculation of 4T1 mice breast cancer |
| 投稿时间：2025-11-30 修订日期：2026-05-09 |
| DOI：10.13929/j.issn.1003-3289.2026.06.002 |
| 中文关键词: 小鼠 乳腺肿瘤 显微超声造影 微循环 |
| 英文关键词:mice breast neoplasms super resolution contrast-enhanced ultrasound microcirculation |
| 基金项目:贵州省科技计划项目(黔科合基础-ZK[2022]一般403)、贵州省卫生健康委科学技术基金(gzwkj2026-431)。 |
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| 中文摘要: |
| 目的 观察基于不同帧频显微超声造影(SR CEUS)评估4T1小鼠乳腺癌微循环的价值。方法 于20只BALB/c雌性小鼠右侧腹股沟区皮下注射4T1小鼠乳腺癌细胞,之后分别行二维超声、CDFI、超声造影(CEUS)及不同帧频下(110、220、330及500 fps)SR CEUS,观察超声表现并分析定量参数。结果 4T1小鼠乳腺癌病灶呈椭圆形,最大径(9.85±1.49)mm;CDFI于瘤周探及少许血流信号;CEUS见病灶不均质高增强;SR CEUS可见瘤体内部血流分布、走行及细微血管。随SR CEUS帧频升高,瘤体血流最大速度(Max Vel)、中值速度(Med Vel)、最大强度(Max Inten)及速度方差(Vel Var)均依次升高(P均＜0.05);220、330及500 fps下血管密度(VD)及分形维数(FD)均低于110 fps(P均＜0.05);500 fps下灌注指数(PI)高于110、220及300 fps(P均＜0.05);330及500 fps下中值强度(Med Inten)均高于110及220 fps(P均＜0.05)。结论 基于不同帧频SR CEUS可通过影响微泡运动的捕捉精度、显示微血管结构能力及量化灌注功能准确性而更全面地反映4T1小鼠乳腺癌微循环的异质性。 |
| 英文摘要: |
| Objective To observe the value of different frame frequency super resolution contrast-enhanced ultrasound (SR CEUS) for evaluating microcirculation of 4T1 mouse breast cancer. Methods 4T1 mouse breast cancer cells were subcutaneously injected into the right groin area of 20 female BALB/c mice. Two-dimensional ultrasound, CDFI, contrast-enhanced ultrasound (CEUS) and SR CEUS at different frame frequencies (110, 220, 330, 500 fps) were performed to observe the ultrasonic manifestations and analyze quantitative parameters. Results The lesions of 4T1 mouse breast cancer were ovally shaped, with the maximum diameter of (9.85±1.49) mm. CDFI detected few blood flow signals around the tumor. CEUS showed heterogeneous hyper-enhancement of lesions. SR CEUS described the blood flow distribution, course and fine vessels in the tumor. With the increase of SR CEUS frame frequency, the maximum velocity (Max Vel), the median velocity (Med Vel), the maximum intensity (Max Inten) and velocity variance (Vel Var) of tumor blood flow increased in sequence (all P＜0.05). The vessel density (VD) and fractal dimension (FD) under 220, 330 and 500 fps were lower than those under 110 fps (all P＜0.05). The perfusion index (PI) under 500 fps was higher than that under 110, 220 and 300 fps (all P＜0.05). Med Inten under 330 and 500 fps was higher than that under 110 and 220 fps (all P＜0.05). Conclusion The combination of SR CEUS with different frame frequencies could more comprehensively reflect the heterogeneity of microcirculation of 4T1 mouse breast cancer through affecting the accuracy of capture of microbubbles motion, the ability for display microvascular structures and the accuracy of quantification of perfusion function. |
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