| 孙维荣,张国辉,姚籴霄,李竞,张啸波,张肖,何晓锋,张欣,魏颖恬,张忠亮,王振军,肖越勇.H22肝癌细胞联合基质胶用于建立小鼠皮下肝癌模型[J].中国医学影像技术,2025,41(12):1976~1979 |
| H22肝癌细胞联合基质胶用于建立小鼠皮下肝癌模型 |
| Combining H22 liver cancer cells and suspension Matrigel for establishing mouse subcutaneous liver cancer model |
| 投稿时间：2025-05-15 修订日期：2025-07-22 |
| DOI：10.13929/j.issn.1003-3289.2025.12.008 |
| 中文关键词: 模型,动物 癌,肝细胞 消融技术 磁共振成像 |
| 英文关键词:models, animal carcinoma, hepatocellular ablation techniques magnetic resonance imaging |
| 基金项目: |
| 作者 | 单位 | E-mail | | 孙维荣 | 中国人民解放军总医院第八医学中心放射诊断科, 北京 100091
中国人民解放军总医院第一医学中心放射诊断科, 北京 100853 | | | 张国辉 | 中国人民武装警察部队特色医学中心放射诊断科, 天津 300162 | | | 姚籴霄 | 齐齐哈尔医学院附属第二医院医学影像中心, 黑龙江 齐齐哈尔 161006 | | | 李竞 | 中国人民武装警察部队特色医学中心放射诊断科, 天津 300162 | | | 张啸波 | 中国人民解放军总医院第一医学中心放射诊断科, 北京 100853 | | | 张肖 | 中国人民解放军总医院第一医学中心放射诊断科, 北京 100853 | | | 何晓锋 | 中国人民解放军总医院第一医学中心放射诊断科, 北京 100853 | | | 张欣 | 中国人民解放军总医院第一医学中心放射诊断科, 北京 100853 | | | 魏颖恬 | 中国人民解放军总医院第一医学中心放射诊断科, 北京 100853 | | | 张忠亮 | 中国人民解放军总医院第一医学中心放射诊断科, 北京 100853 | | | 王振军 | 中国人民解放军总医院第一医学中心放射诊断科, 北京 100853 | | | 肖越勇 | 中国人民解放军总医院第一医学中心放射诊断科, 北京 100853 | xiaoyueyong@vip.sina.com |
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| 中文摘要: |
| 目的 观察利用H22肝癌细胞联合基质胶建立小鼠皮下肝癌模型的价值。方法 将12只雄性BALB/c小鼠随机分为A、B组,分别于其右侧大腿外侧皮下注射含基质胶(A组)或不含基质胶(B组)的H22肝癌细胞混悬液150 μl(约1×107个细胞)。于接种后第7天行MR检查,评估移植成功率及有无转移,测量肿瘤最长径(L)、短径(W),计算肿瘤体积(V=0.5×L×W2)及L/W比值(代表形态规则程度),比较肿瘤T1值,计算变异系数(CV),据以评估肿瘤均质性;采用分级法,根据肿瘤细胞侵犯解剖层次进行量化评分,评估肿瘤局部侵袭能力。之后处死小鼠,观察肿瘤病理表现。结果 2组小鼠均建模成功,观察期内均未出现明显远处转移。组间肿瘤体积差异无统计学意义(t=-0.561、P=0.587),而A组肿瘤体积标准差小于B组(14.92 vs.22.67)。A组5只肿瘤侵入结缔组织、1只侵入肌层表面,B组1只肿瘤侵入肌层表面、5只侵入深层肌层;B组肿瘤侵袭能力强于A组(P＜0.05)。A组肿瘤T1值CV小于B组 。MR T2WI示A组肿瘤形态较规则、边界清晰,T1 mapping伪彩图示瘤体内信号较均匀;B组肿瘤形态欠规则、边界欠清,T1 mapping伪彩图示瘤体内信号欠均匀。结论 以H22肝癌细胞联合基质胶能获得形态较规则、内部较均质、侵袭能力相对较弱的小鼠皮下肝癌模型。 |
| 英文摘要: |
| Objective To explore the value of H22 liver cancer cells suspension combining with Matrigel for establishing mouse subcutaneous liver cancer model. Methods Twelve male BALB/c mice were randomly divided into groups A and B. Subcutaneously injection of 150 μl of H22 liver cancer cells suspension (approximately 1×107 cells) with Matrigel (group A) or without Matrigel (group B) on the lateral side of the right thigh were performed, respectively. On the 7th day after vaccination, MR examination was performed to observe the success rate of transplantation and metastasis or not. The longest diameter (L) and short diameter (W) of the tumor were measured, and tumor volume (V=0.5×L×W2) and morphological regularity (L/W) were calculated. T1 values of the tumor was compared, and the coefficient of variation (CV) was calculated to evaluate the homogeneity of tumor. Then hierarchical grading method was used to quantitatively score the local invasion ability of the tumor based on anatomical level of invasion. Finally the mice were sacrificed, and pathological manifestations of the tumors were observed. Results Mice subcutaneous liver cancer models were successfully established in both group A and B. No significant difference of tumor volume was found between groups (t=-0.561, P=0.587), but the standard deviation of tumor volume of mice in group A was smaller than that in group B (14.92 and 22.67). Five mice were found with tumor invaded the connective tissue and 1 with invaded surface of muscle layer in group A, while 1 mouse was found with tumor invaded surface of muscle layer and 5 with tumor invaded deep muscle layer in group B, and the invasion ability of tumor in group B was stronger than that in group A (P＜0.05). CV of T1 value in group A was lower than that in group B . MR T2WI showed regular tumor morphology in group A, with clear boundaries, and T1 mapping pseudocolor images showed relative even signals inside the tumor in group A, but irregular tumor morphology, unclear boundaries and uniform signals inside tumor in group B. Conclusion Mouse subcutaneous liver cancer model could with relative regular shape, good homogeneity and less invasive could be successful established using H22 liver cancer cells combined with Matrigel. |
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