| 王子博,杜先勤,辛晓红,赵新颜,胡春红,吕文娟.相位对比CT技术用于三维可视化急性肝损伤小鼠模型离体肝内脉管及坏死灶[J].中国医学影像技术,2025,41(10):1648~1652 |
| 相位对比CT技术用于三维可视化急性肝损伤小鼠模型离体肝内脉管及坏死灶 |
| Phase-contrast CT for three-dimensional visualization of in vitro intrahepatic vessels and necrotic foci in mouse model of acute liver injury |
| 投稿时间：2024-12-02 修订日期：2025-06-13 |
| DOI：10.13929/j.issn.1003-3289.2025.10.008 |
| 中文关键词: 小鼠 肝疾病 坏死 相位对比CT |
| 英文关键词:mice liver diseases necrosis phase-contrast computed tomography |
| 基金项目:天津市教委科研计划(2022KJ197)。 |
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| 中文摘要: |
| 目的 观察相位对比CT技术用于三维可视化急性肝损伤(ALI)小鼠模型离体肝内脉管及坏死灶的可行性。方法 将6只清洁级雄性野生型C57BL/6小鼠随机均分为模型组与对照组,每组3只。对模型组饲喂过量对乙酰氨基酚以诱导ALI模型,对照组饲喂等量蒸馏水;24 h后处死小鼠并取出肝脏进行固定、脱水等处理。针对2组离体肝脏采集相位对比CT并重建断层及三维图像。观察相位对比CT显示肝脏轮廓及其内脉管、坏死灶效果,定量分析坏死灶最大直径及体积,并将CT与病理所见进行对照分析。结果 相位对比CT原始投影及断层图像清晰显示离体肝脏轮廓及内部脉管系统。模型组肝内存在、而对照组未见坏死区域,且相位对比CT与病理所见精准对应。相位对比CT三维重建图清晰显示2组肝内门静脉及肝静脉系统。模型组肝脏见不连续点状坏死灶,主要分布于肝静脉周围,其中位最大径18.50 μm、中位体积5 870.11 μm3;对照组肝内未见坏死灶。结论 相位对比CT技术可用于三维可视化ALI小鼠模型离体肝脏内部脉管及坏死灶。 |
| 英文摘要: |
| Objective To explore the feasibility of phase-contrast CT for three-dimensional visualization of in vitro intrahepatic vessels and necrotic foci in mouse model of acute liver injury (ALI). Methods Six clean grade male wild-type C57BL/6 mice were randomly divided into model group and control group (each n=3). The mice in model group were fed with overdose of acetaminophen to induce ALI, while those in control group were fed with the same amount of distilled water. After 24 h, the mice were all sacrificed, the livers were harvested and then fixed and dehydrated. Phase-contrast CT images of in vitro liver were acquired, sectional and three-dimensional images were reconstructed. The effect of phase-contrast CT for displaying the outline of liver and internal vessels and necrotic foci were observed, the maximum diameter and volume of necrotic foci were quantitatively analyzed, and the results of phase-contrast CT were compared with pathological findings. Results The original projection and sectional images of phase-contrast CT clearly showed the outline of in vitro liver and internal vasculatures. Necrotic foci within the liver were found in model group, but not in control group. The findings of phase-contrast CT corresponded accurately to those of pathology. Three-dimensional reconstruction images of phase-contrast CT clearly displayed intrahepatic portal vein system and hepatic vein system in both groups, and discontinuous punctate necrotic foci within the liver were found in model group, mainly distributed around hepatic vein, with a median maximum diameter of 18.50 μm and a median volume of 5 870.11 μm3, but was not observed in control group. Conclusion Phase-contrast CT could be used in three-dimensional visualization of intrahepatic vessels and necrotic foci of in vitro liver in mouse model of ALI. |
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