| 丘丹霞,陈丽萍,胡志文,马穗红,金海,何杨成,冯玉仪,柳建华.裸鼠MCF7乳腺癌多模态超声定量参数与肿瘤微环境酸化程度的相关性[J].中国医学影像技术,2024,40(12):1813~1817 |
| 裸鼠MCF7乳腺癌多模态超声定量参数与肿瘤微环境酸化程度的相关性 |
| Correlations of multimodal ultrasound quantitative parameters and tumor microenvironment acidification of nude mice MCF7 breast cancer |
| 投稿时间：2024-08-01 修订日期：2024-10-04 |
| DOI：10.13929/j.issn.1003-3289.2024.12.002 |
| 中文关键词: 小鼠,裸 乳腺肿瘤 糖酵解 超声检查 |
| 英文关键词:mice, nude breast neoplasms glycolysis ultrasonography |
| 基金项目:国家自然科学基金(82071935)、广东省自然科学基金(2024A1515011596)。 |
|
| 摘要点击次数: 382 |
| 全文下载次数: 117 |
| 中文摘要: |
| 目的 观察裸鼠MCF7乳腺癌超声造影(CEUS)、微血流成像(MFI)及剪切波弹性成像(SWE)定量参数与肿瘤微环境(TME)酸化程度的相关性。方法 将MCF7人乳腺癌细胞注入20只BALB/c-nu裸鼠皮下,以肿瘤生长至150~200 mm3为建模成功。对裸鼠模型行CEUS、MFI及SWE检查,获取相关参数,包括时间-强度曲线(TIC)参数[峰值强度(PI)和曲线下面积(AUC)]、血管指数(VI)及杨氏模量最大值(Emax)、中位数(Emed)和平均值(Emean)。采用荧光淬灭法测量肿瘤pH值,分析其与CEUS、MFI及SWE参数的相关性。结果 裸鼠MCF7乳腺癌组织pH值为6.05~6.43,平均6.27±0.05;且高灌注区pH值显著高于低灌注区(P＜0.001)。TIC PI和AUC、VI均与pH值呈正相关(r=0.661、0.611、0.768,P均＜0.001),Emax、Emed及Emean与pH值呈负相关(r=－0.490、－0.562、－0.571,P均＜0.001)。结论 裸鼠MCF7乳腺癌TIC参数及VI均与TME酸化程度呈正相关,Emax、Emed及Emean则与TME酸化程度呈负相关。 |
| 英文摘要: |
| Objective To observe the correlations of quantitative parameters of contrast-enhanced ultrasound (CEUS), micro-flow imaging (MFI) and shear wave elastography (SWE) with tumor microenvironment (TME) acidification in nude mice MCF7 breast cancer. Methods MCF7 human breast cancer cells were injected subcutaneously into 20 BALB/c-nu nude mice, and modeling was regarded as successful when tumor grew to 150—200 mm3. Then, CEUS, MFI and SWE were performed to obtain relevant parameters, including time-intensity curve (TIC) parameters (peak intensity[PI], area under the curve[AUC]), vessel index (VI) and maximum, median and mean Young's modulus (Emax, Emed, Emean). Tumor pH value was measured using fluorescence quenching method, and its correlation with CEUS, MFI and SWE parameters were analyzed. Results pH value of MCF7 breast cancer tissue in nude mice was 6.05—6.43, with an average of 6.27±0.05, which in high perfusion area was significantly higher than in low perfusion area (P＜0.001). PI and AUC of TIC and VI were all positively correlated with pH value (r=0.661, 0.611, 0.768, all P＜0.001), while Emax, Emed and Emean were negatively correlated with pH value (r=－0.490, －0.562, －0.571, all P＜0.001). Conclusion TIC parameters and VI of MCF7 breast cancer in nude mice were positively correlated with TME acidification, while Emax, Emed and Emean were negatively correlated with TME acidification. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
