| 张静,董诚,李晓莉,徐文坚.动态对比增强MRI评估化学治疗用于兔VX2恶性骨肿瘤模型早期效果[J].中国医学影像技术,2024,40(10):1457~1460 |
| 动态对比增强MRI评估化学治疗用于兔VX2恶性骨肿瘤模型早期效果 |
| Dynamic contrast enhanced MRI for evaluating early efficacy of chemotherapy in rabbit models of VX2 malignant bone tumor |
| 投稿时间：2024-05-20 修订日期：2024-06-18 |
| DOI：10.13929/j.issn.1003-3289.2024.10.001 |
| 中文关键词: 骨肿瘤 肿瘤治疗方案 动物实验 磁共振成像 |
| 英文关键词:bone neoplasms antineoplastic protocols animal experimentation magnetic resonance imaging |
| 基金项目:国家自然科学基金(81671658)。 |
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| 中文摘要: |
| 目的 观察动态对比增强MRI(DCE-MRI)评估化学治疗(简称化疗)用于兔VX2恶性骨肿瘤模型早期效果的价值。方法 以30只实验兔成功建立VX2恶性骨肿瘤模型,以其中14只为化疗组,经静脉注射顺铂7 mg/kg体质量,以另16只为对照组,经静脉注射等量生理盐水。分别于造模2周后(干预前)及干预后3天采集平扫MRI及DCE-MRI,对MRI与病理所见进行点对点对照,获取瘤体区域容积转移常数(Ktrans)、速率常数(Kep)、血管外细胞外容积分数(Ve)及血浆容积分数(Vp),以及微血管密度(MVD)。比较组内干预前、后MRI结果,以及干预后组间MRI结果,以受试者工作特征(ROC)曲线及曲线下面积(AUC)评估各参数判断是否曾接受化疗的效能,分析干预后DCE-MRI各参数与MVD的相关性。结果 化疗组干预前、后平扫MRI所见肿瘤最大径差异无统计学意义(P＞0.05)。相比干预前,化疗组实体瘤区域Ktrans、Kep均降低、对照组均升高(P均＜0.05),而2组Ve及Vp差异均无统计学意义(P均＞0.05)。干预后化疗组Ktrans及Kep值均低于对照组(P均＜0.05),而组间Ve及Vp差异均无明显统计学意义(P均＞0.05)。以Ktrans、Kep、Ve及Vp判断是否曾接受化疗的AUC分别为0.964、0.933、0.317及0.455。干预后化疗组实体瘤区域MVD低于对照组(P＜0.05);2组实体瘤区域Ktrans、Kep值均与MVD呈正相关(r=0.876、0.881,P均＜0.05),而Ve或Vp值与MVD无显著相关性 (r=0.118、0.202,P均＞0.05)。结论 DCE-MRI定量分析参数Ktrans和Kep可较为敏感地反映化疗用于VX2恶性骨肿瘤模型兔早期效果及其MVD变化。 |
| 英文摘要: |
| Objective To observe the value of dynamic contrast enhanced MRI (DCE-MRI) for evaluating the early efficacy of chemotherapy in rabbit models of VX2 malignant bone tumor. Methods Models of VX2 malignant bone tumor were established in 30 rabbits, among which 14 were treated with intravenous cisplatin by 7 mg/kg body mass (chemotherapy group), while the other 16 were injected with equal dose of normal saline (control group). Non-contrast MRI and DCE-MRI were acquired 2 weeks after tumor implantation (before intervention) and 3 days later injection (after intervention), respectively. Point-to-point comparisons were performed between images and pathological findings, and the values of volume transfer constant (Ktrans), rate constant (Kep), extravascular and extracellular volume fraction (Ve), plasma volume fraction (Vp) and microvessel density (MVD) in tumor region were obtained. MRI findings before and after intervention within groups, as well as those after intervention were compared between groups. The efficacy of the above parameters for judging whether the tumor being treated with chemotherapy or not was evaluated with receiver operating characteristic (ROC) curve and the area under the curve (AUC), and the correlations between DCE-MRI parameters after intervention and MVD were analyzed. Results No statistical difference of the maximum diameter of tumors shown on plain MRI was found before and after intervention in chemotherapy group (P＞0.05). Compared with those before intervention, Ktrans and Kep in tumor region decreased in chemotherapy group but increased in control group after intervention (all P＜0.05), while no significant difference of Ve nor Vp was found in both groups (all P＞0.05). Ktrans and Kep in chemotherapy group were lower than those in control group (both P＜0.05), while no significant difference of Ve nor Vp between groups after intervention (both P＞0.05). The AUC of Ktrans, Kep, Ve and Vp for judging whether the tumor treated with chemotherapy or not was 0.964, 0.933, 0.317 and 0.455, respectively. MVD in tumor regions in chemotherapy group was lower than that in control group after intervention (P＜0.05). Ktrans and Kep in tumor region in both groups were positively correlated with MVD (r=0.876, 0.881, both P＜0.05), while Ve and Vp did not significantly correlated with MVD (r=0.118, 0.202, both P＞0.05). Conclusion DCE-MRI quantitative parameters Ktrans and Kep could be used to sensitively observe the early reflection of rabbit models of VX2 malignant bone tumor to chemotherapy and changes of MVD. |
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