| 李宸,刘洋,张海默,高啸天,李振,刘晨萌,巩平,肖培伦,王晓莉.多模态MRI观察电针治疗大鼠脑缺血再灌注后脑白质损伤[J].中国医学影像技术,2023,39(11):1619~1625 |
| 多模态MRI观察电针治疗大鼠脑缺血再灌注后脑白质损伤 |
| Multimodal MRI observation on electroacupuncture for treating white matter injury after cerebral ischemia reperfusion in rats |
| 投稿时间：2023-07-11 修订日期：2023-09-15 |
| DOI：10.13929/j.issn.1003-3289.2023.11.005 |
| 中文关键词: 大鼠 再灌注损伤 白质 电针 磁共振成像 |
| 英文关键词:rats reperfusion injury white matter electroacupuncture magnetic resonance imaging |
| 基金项目:国家自然科学基金项目(82071888)、山东省自然科学基金项目(ZR2020MH074)、山东省中医药科技发展计划项目(2019-0417)、2019年高等学校青创人才引育计划、潍坊市科技发展计划(2022YX042)。 |
| 作者 | 单位 | E-mail | | 李宸 | 潍坊医学院医学影像学院, 山东 潍坊 261053 | | | 刘洋 | 潍坊医学院医学影像学院, 山东 潍坊 261053
潍坊医学院附属医院影像中心, 山东 潍坊 261031 | | | 张海默 | 潍坊医学院医学影像学院, 山东 潍坊 261053 | | | 高啸天 | 潍坊医学院医学影像学院, 山东 潍坊 261053 | | | 李振 | 潍坊医学院医学影像学院, 山东 潍坊 261053
潍坊医学院附属医院影像中心, 山东 潍坊 261031 | | | 刘晨萌 | 潍坊医学院医学影像学院, 山东 潍坊 261053 | | | 巩平 | 潍坊医学院附属医院影像中心, 山东 潍坊 261031 | | | 肖培伦 | 潍坊医学院基础医学院, 山东 潍坊 261053 | | | 王晓莉 | 潍坊医学院医学影像学院, 山东 潍坊 261053
潍坊医学院附属医院影像中心, 山东 潍坊 261031 | wxlpine@163.com |
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| 中文摘要: |
| 目的 基于多模态MRI动态观察电针治疗大鼠脑缺血再灌注后脑白质损伤。方法 将35只大鼠分为假手术(Sham)组(n=5)、脑缺血再灌注损伤(CIRI)组(n=15)及电针组(n=15)并分别予以相应处理;于其后24 h、72 h及5 d采集颅脑T2WI、弥散加权成像及弥散张量成像,重建获取各向异性分数(FA)、Colored FA图。处死大鼠对脑组织行鼠抗 髓鞘碱性蛋白(MBP)/兔抗神经丝蛋白200(NF200)、鼠抗增殖细胞核抗原(PCNA)/兔抗神经胶质抗原2(NG2)免疫荧光双标染色,观察电针对CIRI大鼠脑白质损伤及脑组织内源性少突胶质祖细胞增殖的影响。结果 24 h、72 h及5 d后,电针组脑梗死体积明显小于CIRI组(P均＜0.05);CIRI组及电针组缺血侧纹状体及胼胝体区相对表观弥散系数(rADC)、相对FA(rFA)均低于Sham组(P均＜0.05);电针组纹状体及胼胝体区rADC、rFA均高于CIRI组(P均＜0.05)。5 d后CIRI组及电针组大鼠缺血侧纹状体和胼胝体区MBP、NF200荧光强度(FI)、纹状体区PCNA及NG2均(+)细胞数均小于Sham组(P均＜0.05),电针组缺血侧纹状体和胼胝体区FIMBP、FINF200、纹状体区PCNA及NG2均(+)细胞数均大于CIRI组(P均＜0.05)。结论 电针治疗可减轻CIRI大鼠脑白质损伤、促进PCNA及NG2生成。 |
| 英文摘要: |
| Objective To observe electroacupuncture for treating white matter injury after cerebral ischemia reperfusion based on multimodal MRI. Methods Totally 35 rats were divided into sham operation (Sham) group (n=5), cerebral ischemia reperfusion injury (CIRI) group (n=15), or electroacupuncture group (n=15) and underwent relative managements. Cranial T2WI, diffusion weighted imaging and diffusion tensor imaging were acquired 24 h, 72 h and 5 d after managements, fraction anisotropy (FA) and Colored FA maps were obtained. The rats were then sacrificed, brain tissue were harvested and stained with mouse anti-myelin basic protein (MBP)/rabbit anti-neurofilament protein 200 (NF200) and mouse anti-proliferating cell nuclear antigen (PCNA)/rabbit anti-neuroglial antigen 2 (NG2) immunofluorescence double-label staining, and the effect of electroacupuncture on white matter injury and the proliferation of endogenous oligodendrocyte progenitor cells in the brain tissue of CIRI rats were observed. Results The volume of cerebral infarction in electroacupuncture group was significantly smaller than that in CIRI group 24 h, 72 h and 5 d after managements (all P＜0.05). The relative apparent diffusion coefficient (rADC) and relative FA (rFA) of ischemic striatum and corpus callosum areas in CIRI group and electroacupuncture group were all lower than those in Sham group (all P＜0.05), while of the striatum and corpus callosum areas in electroacupuncture group were all higher than those in CIRI group (all P＜0.05). Five days after managements, the fluorescence intensity (FI) of MBP, NF200 of the ischemic striatum and corpus callosum, the number of cells with positive of PCNA and NG2 in the striatum in CIRI and electroacupuncture group were all lower than those in Sham group (all P＜0.05). FIMBP, FINF200 of the ischemic striatum and corpus callosum, the number of cells with positive of PCNA and NG2 in the striatum of rats in electroacupuncture group were all higher than those in CIRI group (all P＜0.05). Conclusion Electroacupuncture could reduce white matter injury in CIRI rats and promote generation of PCNA and NG2. |
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