| 杨敏,张盛敏,汪许红.新型定量超声技术评估家兔肝纤维化和肝细胞气球样变[J].中国医学影像技术,2022,38(9):1291~1295 |
| 新型定量超声技术评估家兔肝纤维化和肝细胞气球样变 |
| New quantitative ultrasound techniques for evaluation on hepatic fibrosis and hepatocyte ballooning of rabbits |
| 投稿时间：2022-01-08 修订日期：2022-03-24 |
| DOI：10.13929/j.issn.1003-3289.2022.09.003 |
| 中文关键词: 兔 肝硬化 细胞质空泡化 超声检查 |
| 英文关键词:rabbits liver cirrhosis cytoplasmic vacuolization ultrasonography |
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| 摘要点击次数: 1449 |
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| 中文摘要: |
| 目的 评价新型定量超声(QUS)技术诊断家兔肝纤维化和肝细胞气球样变的价值。方法 将30只新西兰大白兔随机分为实验A组(n=12)、实验B组(n=12)及对照组(n=6)，以高脂饲料喂养建立兔肝细胞脂肪变性模型；采用剪切波弹性成像(SWE)、剪切波频散成像(SWD)及声衰减成像技术测量相关参数，根据病理学结果判断家兔肝纤维化分期及肝细胞气球样变程度，与超声参数进行比较并分析其相关性。结果 实验B组2只家兔死亡，22只模型建立成功，实验A组及B组肝脏均发生不同程度纤维化和肝细胞气球样变。肝纤维化分期是SWV的独立影响因素(P<0.05)；F1~3期与F0期家兔剪切波速度(SWV)差异有统计学意义(P<0.05)，而F1~2期与F3期差异无统计学意义(P>0.05)；肝纤维化分期与SWV呈正相关(r=0.74，P<0.05)。肝细胞气球样变分级是频散值的独立影响因素(P<0.01)；B0、B1、B2级肝细胞气球样变家兔频散值两两比较差异均有统计学意义(P均<0.05)；肝细胞气球样变分级与频散值呈正相关(r=0.76，P<0.05)。声衰减系数与肝纤维化分期及肝细胞气球样变分级均无显著相关(P均>0.05)。以SWV诊断轻度(≥ F1期)和重度纤维化(≥ F3期)的曲线下面积(AUC)分别为0.95及0.84；以频散值诊断轻度(≥ B1级)和重度肝细胞气球样变(B2级)的AUC分别为0.94及0.87。结论 SWE判断家兔肝纤维化分期价值较高；SWE可更好地评估肝细胞气球样变程度。 |
| 英文摘要: |
| Objective To explore the value of new quantitative ultrasound (QUS) techniques for diagnosis of hepatic fibrosis and hepatocyte ballooning in rabbits. Methods Thirty New Zealand white rabbits were randomly divided into experimental group A (n=12), experimental group B (n=12) and control group (n=6). Then rabbit models of hepatocyte steatosis were established using high-fat diet. Shear wave elastography (SWE), shear wave dispersion (SWD) and attenuation imaging were used to measure relevant parameters. According to the pathological results, the stage of hepatic fibrosis and the degree of hepatocyte ballooning were judged and compared with the ultrasonic parameters, and the correlations were analyzed. Results Two rabbits in experimental group B died, and the models were successfully established in other 22 rabbits. Different degrees of hepatic fibrosis and hepatocyte ballooning developed in experimental groups A and B. The stage of hepatic fibrosis was an independent factor of shear wave velocity (SWV) (P<0.05), and SWV were significantly different between F1-3 and F0 (P<0.05), but not different between F1-2 and F3 (P>0.05), and the stage of hepatic fibrosis was positively correlated with SWV (r=0.74, P<0.05). The grade of hepatic ballooning degeneration was an independent factor of the dispersion value (P<0.01). The differences of the frequency dispersion values of hepatocyte ballooning in B0, B1 and B2 were significant (all P<0.05). The hepatocyte ballooning variation grading was positively correlated with the dispersion value (r=0.76, P<0.05), while no significant correlation was found between the acoustic attenuation coefficient, the stage of hepatic fibrosis nor the classification of hepatocyte ballooning (both P>0.05). The area under the curve (AUC) of SWV for diagnosis of mild (≥ F1 stage) and severe hepatic fibrosis (≥ F3 stage) was 0.95 and 0.84, respectively, for diagnosis of mild (≥ B1 grade) and severe hepatocyte ballooning (B2 grade) based on dispersion values was 0.94 and 0.87, respectively. Conclusion SWE had higher value in judging hepatic fibrosis staging, while SWD was better for assessing the degree of hepatocyte ballooning in rabbits. |
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