| 郭胜存,张胜叶,户富栋,陈熙,陈魁.超声联合微泡选择性破坏小鼠腹主动脉不稳定斑块内不成熟血管[J].中国医学影像技术,2022,38(8):1127~1133 |
| 超声联合微泡选择性破坏小鼠腹主动脉不稳定斑块内不成熟血管 |
| Ultrasound combined with microbubbles for selective destruction of immature vessels in abdominal aortic unstable plaque of mice |
| 投稿时间：2021-09-16 修订日期：2022-03-27 |
| DOI：10.13929/j.issn.1003-3289.2022.08.002 |
| 中文关键词: 血管疾病 超声疗法 微泡 动脉粥样硬化斑块 小鼠 |
| 英文关键词:vascular diseases ultrasonic therapy microbubbles plaque, atherosclerotic mice |
| 基金项目:国家自然科学基金(81701695)。 |
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| 中文摘要: |
| 目的 观察超声联合微泡选择性破坏小鼠腹主动脉不稳定斑块内不成熟血管的可行性及其对斑块不稳定性的影响。方法 以高脂饮食饲养30只8周载脂蛋白E (ApoE)基因敲除小鼠至30周龄,建立动脉不稳定斑块模型,随机分为实验组(n=12)、未干预组(n=12)及对照组(n=6)。对实验组经鼠尾静脉弹丸式注入0.1 ml微泡后,以超声辐照腹主动脉斑块对应体表区域,并于治疗后24 h和8周后各处死6只小鼠。对未干预组注入0.1 ml微泡后行腹主动脉超声造影,检查结束后处死动物。对照组仅予超声辐照,于8周后处死。对各组腹主动脉斑块行病理检查,分析斑块易损性,计算易损指数和核/帽比值,评估斑块微血管密度(MVD)。对比实验组与未干预组斑块内微血管情况,以及实验组与对照组斑块易损性相关指标。结果 实验组超声联合微泡治疗24 h后斑块内不成熟血管MVD较未干预组降低(P＜0.05);治疗8周后,与对照组比较,实验组超声联合微泡斑块易损性指标改善,斑块MVD减少(P均＜0.05)。未干预组斑块易损指数及核/帽比值与斑块内总MVD和不成熟血管MVD均呈正相关(P均＜0.05),与成熟血管MVD均无明显相关(P均＞0.05)。结论 超声联合微泡可有效选择性破坏小鼠腹主动脉不稳定斑块内的不成熟血管,改善斑块不稳定性。 |
| 英文摘要: |
| Objective To investigate the feasibility of ultrasound combined with microbubbles in selective destruction of immature vessels in abdominal aortic unstable plaque in mice and its impact on plaque instability. Methods Totally 30 apolipoprotein E (ApoE)-deficient mice aged 8 weeks were fed with high-fat diet to 30 weeks for establishment of arterial unstable plaque models. The mice were randomly divided into experimental group (n=12), untreated group (n=12) and control group (n=6). Ultrasonic irradiation was performed on the body surface area corresponding to abdominal aortic plaque after injection of 0.1 ml microbubbles via tail vein of mice in experimental group, then 6 mice were sacrificed 24 h and 8 weeks after treatment, respectively. Contrast-enhanced ultrasound of abdominal aorta was performed after pellet injection of 0.1 ml microbubbles in untreated group, then the mice were sacrificed. Mice in control group underwent only ultrasound irradiation, and were sacrificed 8 weeks after treatment. The abdominal aortic plaques were collected for pathological examinations, the plaque vulnerabilities were evaluated, the vulnerability index and nuclear/cap ratio were calculated, and the plaque microvascular densities (MVD) were evaluated. Microvessels in the plaques in experimental group and untreated group were comparatively observed, and changes of plaque vulnerability related indexes were compared between experimental group and control group. Results Compared with untreated group, the immature vessels MVD in plaques in experimental group decreased 24 h after ultrasound combined with microbubbles (P<0.05). Compared with control group, the plaque vulnerability related indexes improved and plaque MVD decreased in experimental group 8 weeks after treatment (all P<0.05). The vulnerability index and nuclear/cap ratio in untreated group were positively correlated with total MVD and immature vessels MVD in plaques, respectively (all P<0.05), but not obviously correlated with mature vessels MVD (both P>0.05). Conclusion Ultrasound combined with microbubbles could be used to selectively and effectively destroy immature vessels in abdominal aortic unstable plaques and further improve the instability of plaques in mice. |
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