| 骆杰,钟世根,张勇,凌智瑜,王志刚.近红外荧光分子探针用于CT成像及术中导航[J].中国医学影像技术,2022,38(5):641~647 |
| 近红外荧光分子探针用于CT成像及术中导航 |
| Near infrared fluorescence molecular probe for CT imaging and intraoperative navigation |
| 投稿时间：2022-01-05 修订日期：2022-04-30 |
| DOI：10.13929/j.issn.1003-3289.2022.05.001 |
| 中文关键词: 分子探针技术 体层摄影术,X线计算机 立体定位技术 |
| 英文关键词:molecular probe techniques tomography, X-ray computed stereotaxic techniques |
| 基金项目:国家自然科学基金(81571692)。 |
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| 中文摘要: |
| 目的 制备近红外荧光分子探针,观察其用于增强CT成像及术中导航效果。方法 采用薄膜水化法制备载IR780和全氟溴辛烷(PFOB)的脂质体(IR780@LIP-PFOB),检测其基本理化性质,观察其用于增强体内、外CT成像的效果。以激光共聚焦显微镜和流式细胞仪观察4T1细胞对IR780@LIP-PFOB的摄取情况,以活体荧光成像检测IR780@LIP-PFOB在荷4T1瘤BALB/c小鼠体内的分布,通过冰冻切片观察IR780@LIP-PFOB在肿瘤区和周围非瘤组织的分布;以实时体视荧光成像系统评估IR780@LIP-PFOB用于术中导航的效能。结果 所获IR780@LIP-PFOB大小均匀,呈球形,平均粒径(262.80±117.60)nm,表面电位(－20.10±6.38)mV,IR780包封率为82.92%;用于小鼠体内、外CT可显著增强成像效果。IR780@LIP-PFOB可选择性聚集于4T1细胞周围并于肿瘤区富集,而非瘤组织中未见其明显聚集;用于术中导航可清晰辨识肿瘤组织及其边界。结论 成功制备的近红外荧光分子探针用于小鼠能增强CT成像效果,并支持术中导航。 |
| 英文摘要: |
| Objective To prepare a near infrared fluorescent molecular probe, and to explore its role in CT imaging and intraoperative navigation.Methods IR780 dye and perfluorooctyl bromide (PFOB)-based liposomes (IR780@LIP-PFOB) were prepared using the thin film hydration method, and the basic physical and chemical properties were detected, and the in vivo and in vitro CT imaging performances in 4T1 tumor-bearing BALB/c mice were observed. 4T1 cells for IR780@LIP-PFOB uptake were observed with laser scanning confocal microscope and flow cytometry. The biodistribution of IR780@LIP-PFOB was analyzed with fluorescence imaging, while of IR780@LIP-PFOB in the tumor area and surrounding non-tumor tissue was observed through frozen section. Real-time stereoscopic fluorescence imaging system was used to evaluate the role of IR780@LIP-PFOB in intraoperative navigation.Results The synthesized IR780@LIP-PFOB was uniform and spherical, with an average diameter of (262.80±117.60)nm and surface potential of (-20.10±6.38)mV. The loading efficacy of IR780 was 82.92%. IR780@LIP-PFOB could obviously improve CT imaging. IR780@LIP-PFOB selectively aggregated around 4T1 cells, and accumulated in tumor area, but not in surrounding non-tumor tissue. After the administration of IR780@LIP-PFOB, tumor tissue and boundary clearly displayed for intraoperative navigation.Conclusion The prepared fluorescent molecular probe improved in vivo and in vitro CT imaging of BALB/c mice and supported intraoperative navigation. |
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