| 罗静,王培军.多功能复合纳米材料Fe-Cur-KB靶向β淀粉样蛋白:构建与体外MR成像[J].中国医学影像技术,2021,37(4):481~485 |
| 多功能复合纳米材料Fe-Cur-KB靶向β淀粉样蛋白:构建与体外MR成像 |
| Multi-functional nanocomposite Fe-Cur-KB targeting amyloid β-protein: Construction and in vitro MR imaging |
| 投稿时间：2020-05-29 修订日期：2021-02-28 |
| DOI：10.13929/j.issn.1003-3289.2021.04.001 |
| 中文关键词: 阿尔茨海默病 磁性氧化铁纳米粒子 血脑屏障 淀粉样β肽 |
| 英文关键词:Alzheimer disease magnetic iron oxide nanoparticles blood-brain barrier amyloid beta-peptides |
| 基金项目:国家自然科学基金重点项目（81830059）、国家自然科学基金面上项目（81771889）。 |
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| 中文摘要: |
| 目的 构建可特异性结合阿尔茨海默病（AD）病理标志物β淀粉样蛋白（Aβ）的磁性纳米粒子，观察其用于早期诊断AD的潜力。方法 采用溶剂蒸发法构建以Aβ为靶向基团，携带姜黄素（Cur）颗粒、KB探针及四氧化三铁的磁性纳米材料，观察其粒径、形态、水动力粒径和Zeta电位、体外MR成像表现、细胞毒性及透过血脑屏障（BBB）能力，并验证其体外结合Aβ能力。结果 所构建的Fe-Cur-KB磁性纳米材料携带负电荷，Zeta电位约-17.3 mV，水动力粒径约26.93 nm，体外MR显像能力良好。不同浓度Fe-Cur-KB纳米溶液对马-达二氏犬肾（MDCK）细胞存活率无明显影响（P>0.05）。实验组光密度（OD）高于对照组（P<0.05）。碱性磷酸酶与普鲁士蓝共染结果表明，该纳米材料可与Aβ斑块特异性结合。结论 Fe-Cur-KB磁性纳米材料具有良好的生物相容性和稳定性，体外MR显像能力及透过BBB并与Aβ靶向结合能力，可作为MR显像剂用于早期诊断AD。 |
| 英文摘要: |
| Objective To construct magnetic nanomaterials being able to specifically bind to amyloid β-protein (Aβ), which is a pathological marker of Alzheimer disease (AD), and to observe its potential for early diagnosis of AD through in vitro experiments. Methods The solvent evaporation method was used to construct magnetic nanomaterials with Aβ as the target group and carrying curcumin (Cur) particles, KB probe and ferric. The particle size, shape, hydrodynamic size, Zeta potential, MR imaging performance, cytotoxicity and blood-brain barrier penetration were observed, and the ability to bind Aβ was explored through in vitro experiments. Results The constructed Fe-Cur-KB magnetic nanomaterials carried negative charges, the Zeta potential was about -17.3 mV, and the hydrodynamic size was about 26.93 nm, with good in vitro imaging ability. The survival rate of Madin-Darby canine kidney (MDCK) cells were not statistically different among different concentrations of Fe-Cur-KB nano-solutions (P>0.05). The optical density (OD) of the experimental group was higher than that of control group (P<0.05). The co-staining results of alkaline phosphatase and Prussian blue indicated that the nanomaterials can specifically bind to Aβ being plaques. Conclusion The constructed Fe-Cur-KB magnetic nanomaterial had good biocompatibility and stability and in vitro MR imaging ability, which could penetrate BBB and bind to Aβ, being able to be used as MR imaging agent for early diagnosis of AD. |
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