| 赵佳雯,张亮,周頔,王志刚,杨心怡,文明.载硫化铋及阿霉素靶向相变型纳米粒多模态显像及联合治疗卵巢癌体外实验[J].中国医学影像技术,2021,37(1):13~18 |
| 载硫化铋及阿霉素靶向相变型纳米粒多模态显像及联合治疗卵巢癌体外实验 |
| Multi-modal imaging of targeted phase-change nanoparticles containing bismuth sulfide and doxorubicin and combined treatment of ovarian cancer in vitro |
| 投稿时间：2019-12-05 修订日期：2020-06-17 |
| DOI：10.13929/j.issn.1003-3289.2021.01.003 |
| 中文关键词: 硫化铋 阿霉素 光化学疗法 多模态成像 |
| 英文关键词:bismuth sulfide doxorubicin photochemotherapy multimodal imaging |
| 基金项目:国家自然科学基金青年科学基金项目（81801717）、重庆医科大学附属第一医院医学科学培育基金（PYJJ2018-15）。 |
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| 中文摘要: |
| 目的 制备叶酸靶向载硫化铋（Bi2S3）与阿霉素（DOX）相变型纳米粒（FBS-PFP-PLGA@DOX），观察其基本性能、体外多模态显像及光热联合治疗体外卵巢癌的效果。方法 以双乳化法制备FBS-PFP-PLGA@DOX，检测其理化性质；观察其体外多模态显像；采用近红外激光辐照FBS-PFP-PLGA@DOX并实时记录其温度变化；以细胞计数试剂盒（CCK-8）评估光热联合治疗对卵巢癌细胞（SKOV-3）的体外杀伤作用。结果 所制备FBS-PFP-PLGA@DOX平均粒径为（255.00±90.82） nm，平均电位为（-7.07±4.33） mV，DOX及Bi2S3包封率分别为（26.77±0.46）%和（87.57±0.12）%。体外实验中，FBS-PFP-PLGA@DOX浓度越高，多模态显像效果越好。激光照射后FBS-PFP-PLGA@DOX迅速升温，并呈浓度依赖性。FBS-PFP-PLGA@DOX纳米粒与SKOV-3细胞孵育并辐照激光后细胞存活率为12.17%，杀伤肿瘤细胞能力高于其余各组。结论 所制备FBS-PFP-PLGA@DOX具备体外多模态显像和联合治疗卵巢癌能力。 |
| 英文摘要: |
| Objective To prepare folate-targeted bismuth sulfide (Bi2S3) and doxorubicin (DOX) phase-change poly(lactic co glycolic acid) (PLGA) nanoparticles (FBS-PFP-PLGA@DOX), and to observe its basic performance, multi-modal imaging and therapeutic effects of photothermal combined with chemotherapy for ovarian cancer in vitro. Methods FBS-PFP-PLGA@DOX were prepared by using double emulsion solvent evaporation method (W/O/W). The basic physicochemical properties and multi-modal imaging effects of FBS-PFP-PLGA@DOX were detected. The temperature variation was recorded in real time by using irradiating FBS-PFP-PLGA@DOX with near-infrared laser. The cell killing effects of photothermal therapy on ovarian cancer cell (SKOV-3) combined with chemotherapy were evaluated with Cell Counting Kit-8 (CCK-8). Results The average particle size of prepared FBS-PFP-PLGA@DOX was (255.00±90.82)nm, and the average zeta potential of FBS-PFP-PLGA@DOX nanoparticles was (-7.07±4.33)mV. The entrapment efficiency of DOX and Bi2S3 was (26.77±0.46)% and (87.57±0.12)%, respectively. The multi-modal imaging effects gradually enhanced with the increasing concentration of FBS-PFP-PLGA@DOX. After near-infrared laser irradiation, the nanoparticles were rapidly warmed up in a concentration-dependent manner. The cell survival rate of FBS-PFP-PLGA@DOX after laser irradiation was 12.17%, killing tumor cells better than the other groups. Conclusion The prepared FBS-PFP-PLGA@DOX had the ability of multi-modal imaging and combined treatment of ovarian cancer in vitro. |
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