| 彭一檬,余录,谭华,尤强,张春银.Micro PET/CT观察三七总皂苷对脑缺血再灌注损伤大鼠模型脑葡萄糖代谢的影响[J].中国医学影像技术,2020,36(12):1766~1770 |
| Micro PET/CT观察三七总皂苷对脑缺血再灌注损伤大鼠模型脑葡萄糖代谢的影响 |
| Micro PET/CT observation on effects of Panax notoginseng total saponins on cerebral glucose metabolism in rat models of cerebral ischemia reperfusion injury |
| 投稿时间：2019-11-22 修订日期：2020-12-11 |
| DOI：10.13929/j.issn.1003-3289.2020.12.002 |
| 中文关键词: 再灌注损伤 正电子发射断层显像 体层摄影术,X线计算机 18F氟脱氧葡萄糖 大鼠 三七总皂苷 葡萄糖转运蛋白 |
| 英文关键词:reperfusion injury positron-emission tomography tomography, X-ray computed fluorodeoxyglucose F18 rats Panax notoginseng total saponins glucose transporter |
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| 中文摘要: |
| 目的 采用Micro PET/CT观察经三七总皂苷（PNS）治疗后脑缺血再灌注损伤大鼠模型脑葡萄糖代谢动态变化，探讨PNS影响葡萄糖代谢的机制。方法 将40只SD大鼠随机分为正常组、模型组、假手术组及PNS组，每组10只，以线栓法建立脑缺血再灌注模型。观察造模成功后2、6、24、48及72 h各组Micro PET/CT图像，测定感兴趣容积（VOI）的标准摄取值（SUV）；对比术后72 h各组大鼠脑组织葡萄糖转运蛋白1（GLUT-1）和葡萄糖转运蛋白3（GLUT-3）表达。结果 正常组及假手术组各时间点脑组织SUV差异均无统计学意义（P均>0.05）。PNS组术后6、24、48及72 h SUV均明显高于模型组（P均<0.05），术后6 h脑缺血灶糖代谢水平最低，术后24 h、48 h及72 h不同程度恢复。模型组及PNS组GLUT-1、GLUT-3平均光密度值高于正常组及假手术组，且PNS组高于模型组（P均<0.05）。结论 PNS可通过影响GLUT-1及GLUT-3表达而提高脑缺血再灌注损伤大鼠葡萄糖代谢，起到神经保护作用。 |
| 英文摘要: |
| Objective To observe dynamic changes of cerebral glucose metabolism in rat models of cerebral ischemia reperfusion injury after treatment with Panax notoginseng total saponins (PNS) with Micro PET/CT, and to explore the mechanism of PNS affecting glucose metabolism. Methods A total of 40 SD rats were randomly divided into normal group, model group, sham operation group and PNS group (each n=10). Cerebral ischemia reperfusion models were established using suture occluded method. Micro PET/CT images were observed 2 h, 6 h, 24 h, 48 h and 72 h after successfully modeling, and standardized uptake of value (SUV) in the volume of interest (VOI) were measured. The expression of glucose transporter 1 (GLUT-1) and glucose transporter 3 (GLUT-3) in brain tissue 72 h after operation were compared among groups. Results There was no statistically difference of brain tissue SUV between normal group and sham operation group at all time points (all P>0.05). SUV of PNS group was significantly higher than that of model group 6 h, 24 h, 48 h and 72 h after operation (all P<0.05). Glucose metabolism level of cerebral ischemia became the lowest 6 h after operation, and then recovered with varying degrees 24 h, 48 h and 72 h after operation. The mean optical density values of GLUT-1 and GLUT-3 in model group and PNS group were higher than those in normal group and sham operation group, and in PNS group were higher than those in model group (all P<0.05). Conclusion PNS could improve glucose metabolism of cerebral ischemia reperfusion injury rats, playing an neuroprotective role through affecting the expression of GLUT-1 and GLUT-3. |
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