| 孙智超,王飞,张运帷,王爽,于海源,沈文茜,陈双,吴言,郭延辉,田家玮,杜国庆.基于中智相似积分算法心肌超声造影系统自动定量分析缺血再灌注大鼠存活心肌[J].中国医学影像技术,2020,36(4):508~513 |
| 基于中智相似积分算法心肌超声造影系统自动定量分析缺血再灌注大鼠存活心肌 |
| Automatic quantitative assessment of viable myocardium after ischemia-reperfusion using myocardial contrast echocardiography based on neutrosophic similarity score system |
| 投稿时间：2019-12-25 修订日期：2020-02-25 |
| DOI：10.13929/j.issn.1003-3289.2020.04.007 |
| 中文关键词: 超声心动描记术 心肌再灌注 中智相似积分 计算机辅助 |
| 英文关键词:echocardiography myocardial reperfusion neutrosophic similarity score computer-aided |
| 基金项目:国家自然科学基金（81671762）、黑龙江省博士后启动基金（LBH-Q16144）、哈尔滨医科大学研究生科研和实践创新项目（YJSSJCX2018-73HYD）。 |
| 作者 | 单位 | E-mail | | 孙智超 | 哈尔滨医科大学附属第二医院超声科, 黑龙江 哈尔滨 150001
哈尔滨医科大学心肌缺血机理与诊疗技术省部共建教育部重点实验室, 黑龙江 哈尔滨 150001 | | | 王飞 | 哈尔滨医科大学附属第二医院超声科, 黑龙江 哈尔滨 150001
哈尔滨医科大学心肌缺血机理与诊疗技术省部共建教育部重点实验室, 黑龙江 哈尔滨 150001 | | | 张运帷 | 哈尔滨医科大学附属第二医院超声科, 黑龙江 哈尔滨 150001
哈尔滨医科大学心肌缺血机理与诊疗技术省部共建教育部重点实验室, 黑龙江 哈尔滨 150001 | | | 王爽 | 哈尔滨医科大学附属第二医院超声科, 黑龙江 哈尔滨 150001
哈尔滨医科大学心肌缺血机理与诊疗技术省部共建教育部重点实验室, 黑龙江 哈尔滨 150001 | | | 于海源 | 哈尔滨医科大学附属第二医院超声科, 黑龙江 哈尔滨 150001
哈尔滨医科大学心肌缺血机理与诊疗技术省部共建教育部重点实验室, 黑龙江 哈尔滨 150001 | | | 沈文茜 | 哈尔滨医科大学附属第二医院超声科, 黑龙江 哈尔滨 150001
哈尔滨医科大学心肌缺血机理与诊疗技术省部共建教育部重点实验室, 黑龙江 哈尔滨 150001 | | | 陈双 | 哈尔滨医科大学附属第二医院超声科, 黑龙江 哈尔滨 150001
哈尔滨医科大学心肌缺血机理与诊疗技术省部共建教育部重点实验室, 黑龙江 哈尔滨 150001 | | | 吴言 | 哈尔滨医科大学附属第二医院超声科, 黑龙江 哈尔滨 150001
哈尔滨医科大学心肌缺血机理与诊疗技术省部共建教育部重点实验室, 黑龙江 哈尔滨 150001 | | | 郭延辉 | 美国伊利诺伊大学斯普林菲尔德分校计算机系, 美国 伊利诺伊 62703 | | | 田家玮 | 哈尔滨医科大学附属第二医院超声科, 黑龙江 哈尔滨 150001
哈尔滨医科大学心肌缺血机理与诊疗技术省部共建教育部重点实验室, 黑龙江 哈尔滨 150001 | | | 杜国庆 | 哈尔滨医科大学附属第二医院超声科, 黑龙江 哈尔滨 150001
哈尔滨医科大学心肌缺血机理与诊疗技术省部共建教育部重点实验室, 黑龙江 哈尔滨 150001 | duguoqing9@163.com |
|
| 摘要点击次数: 3444 |
| 全文下载次数: 999 |
| 中文摘要: |
| 目的 观察基于中智相似积分（NSS）算法的心肌超声造影（MCE）分析系统自动定量评估缺血再灌注大鼠存活心肌的准确性。方法 将12只SD大鼠制成心肌缺血再灌注（I/R）模型，随机分为缺血30 min再灌注组（I/R-30组）及缺血45 min再灌注组（I/R-45组），每组6只。分别于术前、阻断即刻、再灌注后7、14及28天行MCE，获取左心室短轴（乳头肌水平）切面图像，采用MCE-NSS系统自动勾勒心内膜及心外膜边界，并将心肌均分为18个节段，获得各心肌节段室壁增厚率（WT）及标化造影剂灌注强度（CI）值。阻断即刻将室壁收缩运动减弱或消失节段（WT<0.3）定义为危险节段；再灌注后危险节段划分为3个区域：WT<0.3且CI<-54 Pix为危险中央区，WT<0.3且CI>-54Pix为危险周边区，WT>0.3且CI>-54Pix为危险恢复区；观察阻断即刻及术后7、14、28天危险中央区、周边区和恢复区面积变化情况。术后28天取大鼠心脏行Masson染色及免疫组织化学检查，计算梗死面积和微血管密度（MVD），分析其与MCE-NSS系统测得梗死面积的相关性。结果 ①危险节段各区域面积变化：术后7、14和28天，2组危险中央区面积百分比变化与阻断即刻变化无明显差异（P均>0.05），而危险周边区面积逐渐减小（P均<0.01），危险恢复区面积逐渐增加（P均<0.01）；I/R-45组危险中央区面积各时间点均大于I/R-30组（P均<0.01），2组危险周边区和危险恢复区面积差异均无统计学意义（P均>0.05）。②与病理结果比较：危险中央区面积与Masson染色测得梗死面积呈正相关（r=0.81，P<0.01），危险周边区CI值与免疫组织化学测得MVD呈正相关（r=0.86，P<0.01）。结论 新型MCE-NSS系统可评估大鼠心肌I/R后左心室局部收缩功能及微循环情况，并能识别存活心肌。 |
| 英文摘要: |
| Objective To explore the accuracy of automatic quantitative assessment for viable myocardium of rat ischemic-reperfusion (I/R) models using myocardial contrast echocardiography (MCE) based on neutrosophic similarity score (MCE-NSS) system. Methods Totally 12 SD rats underwent 30 min (I/R-30 group, n=6) or 45 min (I/R-45 group, n=6)occlusion of the left anterior descending coronary artery followed by reperfusion. MCE was performed before and immediately, 7, 14 and 28 days after operation. The left ventricular myocardium was divided into 18 segments, and the standardized contrast intensity (CI) and wall thickness (WT) of each myocardial segment were automatically calculated using MCE-NSS system after identifying the endocardial and epicardial boundary. The segments with WT<0.3 were considered as the dangerous segments during occlusion, and then the dangerous central region (WT<0.3 and CI<-54 Pix), peripheral region (WT<0.3 and CI>-54 Pix) and recovered region (WT>0.3 and CI>-54 Pix) were defined by MCE-NSS system after reperfusion. The changes of the central areas, peripheral areas and recovery areas were observed immediately, 7, 14 and 28 days after operation. The heart of rats were harvested 28 days after I/R, and myocardial sections were stained with Masson staining and immunohistochemistry to calculation of infarct area and microvessel density (MVD), and the correlation between which and the infarct area measured by MCE-NSS system were analyzed. Results ①For area changes of dangerous sections, no significant difference of the dangerous central region was found between 2 groups immediately and 7, 14 and 28 days after operation (all P>0.05), while the dangerous peripheral region gradually decreased and the recovered region gradually increased 7, 14 and 28 days after reperfusion(all P<0.01). The areas of the dangerous central region in I/R-45 group were all significantly larger than in I/R-30 group (all P<0.01), but there was no significant difference of the dangerous peripheral and recovered region between 2 groups (all P>0.05). ②The areas of the dangerous central regions were positively correlated with infarct size calculated by Masson staining (r=0.81, P<0.01), and the CI value of the dangerous peripheral regions was positively correlated with MVD obtained by immunohistochemistry (r=0.86, P<0.01). Conclusion MCE-NSS system can be used to automatically and quantitatively assess the regional systolic function and microcirculation in left ventricle, and then identify viable myocardium of rats after I/R. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
