王梦辰,刘铁利,蒋玉涵,韩亮,王微微,苗延巍.SWI评估2型糖尿病模型兔海马组织β淀粉样蛋白异常沉积[J].中国医学影像技术,2019,35(8):1159~1163 |
SWI评估2型糖尿病模型兔海马组织β淀粉样蛋白异常沉积 |
SWI in evaluation of abnormal β-amyloid protein deposition in hippocampus of rabbits with type 2 diabetes mellitus |
投稿时间:2019-01-07 修订日期:2019-06-25 |
DOI:10.13929/j.1003-3289.201901047 |
中文关键词: 糖尿病,2型 淀粉样蛋白 海马 磁共振成像 |
英文关键词:diabetes mellitus,type 2 amyloid hippocampus magnetic resonance imaging |
基金项目:国家自然科学基金(81671646、81801657)。 |
|
摘要点击次数: 3282 |
全文下载次数: 908 |
中文摘要: |
目的 探讨SWI评估2型糖尿病(T2DM)模型兔海马组织β淀粉样蛋白(Aβ)异常蓄积的可行性。方法 将15只兔随机分为T2DM组10只、对照组5只,T2DM组给予链脲佐菌素制备T2DM模型,对照组常规喂养,分别于造模成功(n=7)后第2、6、10周末行MR扫描,获得右侧海马区SWI相位值。在第10周末取2组兔右侧海马组织行Aβ1-42免疫组织化学染色,检测Aβ1-42平均光密度值,分析T2DM兔右侧海马区SWI相位值与海马组织Aβ1-42表达的相关性。结果 2组兔第2、6、10周末右侧海马区相位值均逐渐减低。T2DM组第2、6、10周末右侧海马区相位值均低于对照组,仅第10周末组间差异有统计学意义(P<0.01)。T2DM组右侧海马组织Aβ1-42平均光密度值高于对照组(P<0.01)。T2DM兔右侧海马区相位值与海马组织Aβ1-42表达呈负相关(r=-0.88,P<0.01)。结论 SWI可反映T2DM兔脑组织Aβ异常蓄积。 |
英文摘要: |
Objective To explore the feasibility of SWI in evaluating abnormal β-amyloid protein (Aβ) deposition in hippocampus of rabbits with type 2 diabetes mellitus (T2DM). Methods Totally 15 rabbits were randomly divided into T2DM group (n=10) and control group (n=5). In T2DM group, streptozotocin was given to establish T2DM model, while rabbits in control group were given conventional feeding. MR scanning were performed at the end of the 2nd, 4th and 10th week after successful modeling (n=7), and SWI phase values in the right hippocampus were obtained. At the end of the 10th week, the right hippocampal tissue of the two groups of rabbits were taken for immunohistochemical staining of Aβ1-42, and the average optical density of Aβ1-42 was measured. The correlation between the average optical density of Aβ1-42 and the phase value was analyzed. Results The phase values in right hippocampus of both groups decreased gradually. The phase values of T2DM group were lower than that of control group at 2nd, 4th and 10th week each time, while statistically significant difference was only observed at the end of the 10th week (P<0.01). The mean optical density of Aβ1-42 in right hippocampus of T2DM group was higher than that of control group (P<0.01). Correlation analysis showed negative correlation between phase value and Aβ1-42 experssion in right hippocampus of T2DM rabbits (r=-0.88, P<0.01). Conclusion SWI can reflect abnormal deposition of Aβ in brain of T2DM rabbit models. |
查看全文 查看/发表评论 下载PDF阅读器 |