| 马琳,杨蕗璐,陈克霏,王红,卢强,凌文武,罗燕.大鼠70%肝切除后不同程度门静脉狭窄门静脉流速比值[J].中国医学影像技术,2018,34(6):812~816 |
| 大鼠70%肝切除后不同程度门静脉狭窄门静脉流速比值 |
| Portal venous velocity ratios in different degrees of portal vein stenosis following 70% partial hepatectomy of rats |
| 投稿时间：2017-07-20 修订日期：2018-03-21 |
| DOI：10.13929/j.1003-3289.201707095 |
| 中文关键词: 大鼠 肝 肝切除术 门静脉 超声检查 血流速度 |
| 英文关键词:Rats Liver Hepatectomy Portal vein Ultrasonography Blood flow velocity |
| 基金项目:国家自然科学基金面上项目（81071163、81371556）。 |
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| 中文摘要: |
| 目的 观察大鼠70%肝切除后不同程度门静脉狭窄（PVS）模型门静脉血流速度（PVV）比值（PVVR）变化。方法 根据手术方法将102只大鼠随机分为对照组（n=6）、无PVS组及轻、中、重度PVS组（每组各24只）。对无PVS组大鼠仅行70%肝脏切除而不结扎门静脉，轻、中、重度PVS组则在部分肝脏切除基础上不同程度结扎门静脉。于术后1、3、7、14天超声检测PVV，PVS组测量并计算PVVR，结合术后3天核分裂指数（MI）和7天肝再生度（LRD），分析各组PVVR变化。结果 无PVS组及中度PVS组MI高于轻度PVS组（P均<0.05），重度PVS组MI明显低于中度PVS组；重度PVS组LRD明显低于无PVS组和中度PVS组（P均<0.05）。无PVS组术后3天PVV降至最小，7、14天回升。不同程度PVS组大鼠术后7天PVVR下降至最小，14天时有所回升，且术后1天和3天重度组明显大于轻、中度组（P均<0.05），术后7天和14天各组间PVVR差异均无统计学意义（P均>0.05）。结论 超声可准确观察大鼠部分肝脏切除术后PVS血流改变；推测大鼠PVVR变化可能与肝细胞病理改变、核分裂状态、再生肝脏体积等因素有关。 |
| 英文摘要: |
| Objective To analyze the changes of portal venous velocity (PVV) ratio (PVVR) in different degrees of portal vein stenosis (PVS) following 70% partial hepatectomy (PH) in rats. Methods According to different surgical methods, 102 SD rats were randomly subjected into sham operation rats group (n=6), non-PVS group, mild, moderate and severe PVS group (each group n=24). Models of 70% PH without PV ligation were established in non-PVS group, while in PVS groups were produced with partially ligating PV in different degrees following 70% PH. PVV was measured 1, 3, 7 and 14 days after operation, and PVVR was calculated in PVS groups. Combined with mitotic index (MI) 3 days after operation and liver regeneration degree (LRD) 7 days after operation, the changes of PVVR in different groups were analyzed. Results MI of non-PVS and moderate PVS groups were significantly higher than that of mild PVS group (both P<0.05), while of severe PVS group was significantly lower than that of moderate PVS group (P<0.05). LRD of severe PVS group was significantly lower than that of non-PVS and moderate PVS groups (both P<0.05). In non-PVS group, PVV decelerated to the lowest 3 days after operation, and recovered 7 and 14 days. In PVS groups, PVVR decelerated to the lowest 7 days after operation, and recovered until 14 days. PVVR of severe PVS group was significantly higher than that of mild and moderate PVS groups 1 and 3 days after operation (all P<0.05), while there was no statistical differences among PVS groups 7 and 14 days after operation (all P>0.05). Conclusion Ultrasound can well demonstrate blood flow changes of PVS following 70% PH in rats. Changes of PVVR may relate to pathological changes in hepatocytes, the nuclear division and the volume of regenerated liver in rats. |
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