| 李学义,王伟,韩鸿宾,和清源,石春彦,王艾博,滕泽.采用磁共振示踪法探讨大鼠脑细胞间隙内物质转运清除规律[J].中国医学影像技术,2018,34(1):1~4 |
| 采用磁共振示踪法探讨大鼠脑细胞间隙内物质转运清除规律 |
| Tracer-based MRI evaluation on transportation and clearance in extracellular space of rats |
| 投稿时间：2017-11-02 修订日期：2017-12-17 |
| DOI：10.13929/j.1003-3289.201711015 |
| 中文关键词: 脑 磁共振成像 细胞间隙 示踪剂 大鼠 |
| 英文关键词:Brain Magnetic resonance imaging Extracellular space Tracer Rats |
| 基金项目:国家杰出青年科学基金（61625102）、国家自然科学基金（81471633）、北京市科技计划项目基金（Z161100000116041）。 |
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| 中文摘要: |
| 目的 采用磁共振示踪法探讨脑细胞间隙（ECS）内物质转运规律及外界刺激对其转运能力的影响。方法 将32只雄性SD大鼠随机分为尾状核-对照组、丘脑-对照组、尾状核-运动组和丘脑-疼痛组，通过立体定位技术将示踪剂Gd-DTPA导引至尾状核和丘脑区的ECS，在示踪剂注射前和注射后不同时间点进行MR扫描，直至Gd-DTPA所致的高信号消失，通过图像后处理和数学建模技术，计算示踪剂在ECS内的半衰期，采用独立样本t检验比较各组结果。结果 尾状核ECS内的Gd-DTPA可转运至邻近皮层区，丘脑ECS内Gd-DTPA的转运局限于原位，未观察到向邻近区域进行跨区域转运。尾状核-对照组、丘脑-对照组Gd-DTPA的半衰期分别为（104.30±54.12） min和（49.93±2.11） min （t=2.839，P<0.05）。尾状核-运动组Gd-DTPA的半衰期分别为（113.42±47.32） min，与尾状核-对照组比较差异无统计学意义（t=0.359，P>0.05）。丘脑-疼痛组的Gd-DTPA的半衰期为（109.40±10.33） min，较丘脑-对照组显著延长（t=15.954，P<0.05）。结论 磁共振示踪法是研究脑ECS内物质转运规律的有效手段，外界刺激可调控相关脑区ECS内物质的转运清除。 |
| 英文摘要: |
| Objective To explore the rule of transportation in extracellular space (ECS) and the changes induced by the external stimulation with tracer-based MRI.Methods Thirty-two mature Sprague-Dawley rats were randomly divided into four groups,i.e.caudate nucleus-control (Cc) group,thalamus-control (Tc) group,caudate nucleus-moving (Cm) group and thalamus-pain stimulation (Tp) group.The rats were anesthetized and a series of MR scanning were performed before and after the injection of Gd-DTPA in ECS of caudate nucleus and thalamus until the intensity of Gd-DTPA was invisible.Half-life of Gd-DTPA in ECS was calculated and analyzed with t-test.Results Gd-DTPA in caudate nucleus was transported to the ipsilateral cortices away from the injection points,which only distributed on site in the thalamus.The transportation between the two partitions was not observed.The half-life of Tc group ([49.93±2.11]min) was significantly shorter than that of Cc group ([104.30±54.12];t=2.839,P<0.05),no difference was observed between the Cm group ([113.42±47.32]min) and Cc group (t=0.359,P>0.05),while half-life of Tp group ([109.40±10.33]min) was significantly longer than that of Tc group (t=15.954,P<0.05).Conclusion Tracer-based MRI can be used to investigate the rule of transportation in ECS,and the transportation and clearance of substances into the ECS can be influenced by a selective external stimulation. |
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