| 刘影,何文,李少武,邹丽丽,马军.大鼠局灶性脑创伤早期的DTI和1H-MRS的特征[J].中国医学影像技术,2016,32(9):1320~1324 |
| 大鼠局灶性脑创伤早期的DTI和1H-MRS的特征 |
| DTI and 1H-MRS features of early changes in focal traumatic brain injury rat models |
| 投稿时间：2016-02-27 修订日期：2016-07-31 |
| DOI：10.13929/j.1003-3289.2016.09.002 |
| 中文关键词: 脑损伤 扩散张量成像 磁共振波谱成像 动物实验 |
| 英文关键词:Brain injuries Diffusion tensor imaging Magnetic resonance spectroscopy Animal experimentation |
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| 摘要点击次数: 1824 |
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| 中文摘要: |
| 目的 探讨大鼠局灶性创伤性脑损伤（TBI）早期创伤灶周围区的DTI和1H-MRS参数随时间的变化。方法 健康雄性SD大鼠24只，随机分为创伤组16只、对照组8只。创伤组制作大鼠液压冲击脑皮质损伤动物模型，于两组模型制作后1、3、5 h行DTI和1H-MRS扫描，分别测量伤侧病灶周围区及健侧半球对应部位的DTI参数ADC值、FA值和1H-MRS参数N-乙酰天门冬氨（NAA）/肌酸（Cr）、胆碱类化合物（Cho）/Cr和乳酸（Lac）/Cr，对照组测量双侧大脑半球相应部位各指标，并进行统计学分析。结果 创伤组伤后1、3、5h创伤侧病灶周围区与健侧对应部位比较，ADC值均降低、FA值均升高（P均<0.05）；NAA/Cr均降低、Lac/Cr均升高（P均<0.05），Cho/Cr差异无统计学意义（P均>0.05）。创伤灶周围区伤后3h较1h的ADC值降低（P<0.001）、FA值升高（P=0.002），伤后5 h较3 h的ADC值升高（P=0.005）、FA值降低（P=0.012）。对照组双侧大脑半球各时间点各指标差异均无统计学意义（P均>0.05）。结论 大鼠脑皮质局灶性损伤后1~5h创伤灶周围区存在微观结构及代谢变化，创伤灶周围区的继发性脑损伤于伤后1h已发生，而伤后3h可能有所加重。 |
| 英文摘要: |
| Objective To analyze DTI and 1H-MRS changes in perilesional region following focal traumatic brain injury (TBI) in rats. Methods Twenty-four healthy male SD rats were randomly divided into TBI group (n=16) and control group (n=8). Traumatic rat models were made in TBI group with hydraumatic cortical injury. The rats in two groups underwent DTI and 1H-MRS scanning at 1h, 3h, and 5 h after models established. ADC value, FA value, N-acetylaspartate (NAA)/creatine (Cr), choline-containing compounds (Cho)/Cr and lactate (Lac)/Cr were measured in perilesional region (including cortex, external capsule, hippocampus and corpus callosum) and the corresponding parts in contralateral hemisphere. The control group were measured in the corresponding regions in bilateral hemisphere. The statistical analysis was performed. Results ADC values significantly decreased, FA increased, NAA/Cr decreased and Lac/Cr increased in perilesional regions compared with contralateral hemisphere at three time points (all P<0.05). Cho/Cr had no statistical significant difference (P>0.05) at three time points. ADC decreased (P<0.001) and FA increased (P=0.002) in perilesional regions compared 1 h with 3 h after hydraumatic cortical injury. ADC increased (P=0.005) and FA decreased (P=0.012) compared 3 h with 5 h after hydraumatic cortical injury. There were no statistical differences in all parameters of contral group (all P>0.05). Conclusion Microstructural and metabolic changes exist from 1 h to 5 h in perilesional region in rats after TBI, secondary brain injury may occur at 1 h, which become worse at 3 h after trauma. |
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