| 刘莹莹,徐金锋,严飞,曾欣欣,魏章洪,刘慧玉,郑静,周玉丽.Integrin αvβ3靶向超声微泡的制备及体外寻靶实验[J].中国医学影像技术,2016,32(2):175~178 |
| Integrin αvβ3靶向超声微泡的制备及体外寻靶实验 |
| Preparation of Integrin αvβ3 targeted ultrasound microbubbles and targeting study in vitro |
| 投稿时间：2015-07-13 修订日期：2015-12-07 |
| DOI：10.13929/j.1003-3289.2016.02.004 |
| 中文关键词: 整合素αvβ3 造影剂 超声检查 靶向 微泡 |
| 英文关键词:Integrin alphaVbeta3 Contrast media Ultrasonography Targeted Microbubbles |
| 基金项目:广东省自然科学基金项目(2014A030313760)、深圳市科技创新委员会重大项目(JCYJ20130402092657773)。 |
|
| 摘要点击次数: 2725 |
| 全文下载次数: 722 |
| 中文摘要: |
| 目的 制备Integrin αvβ3靶向超声微泡,对其一般理化性质及体外寻靶能力进行检测。方法 采用巯基-马来酰亚胺连接法制备携FITC标记iRGD肽的Integrin αvβ3靶向微泡(MBIntegrin αvβ3),并制备空白微泡(MBcontrol)。检测两组微泡的一般理化性质,在荧光显微镜下观察两组微泡荧光显像,流式细胞仪检测两组微泡FITC荧光含量。于光学显微镜及荧光显微镜下观察两组微泡与小鼠脑微血管内皮细胞(bEnd.3)的黏附情况。结果 ①MBIntegrin αvβ3粒径为(0.84±0.26)μm,与MBcontrol粒径(0.79±0.19)μm差异无统计学意义(P>0.05);②荧光显微镜下观察,MBcontrol组微泡无荧光显示,MBIntegrin αvβ3组微泡表面显示绿色荧光;③流式细胞仪测得MBIntegrin αvβ3组微泡FITC荧光含量为98.4%,MBcontrol组微泡FITC荧光含量为2.5%;④体外寻靶实验显示,MBIntegrin αvβ3组微泡能聚集结合于bEnd.3细胞表面。结论 成功制备了携iRGD肽的Integrin αvβ3靶向微泡,其具有良好的体外寻靶能力。 |
| 英文摘要: |
| Objective To prepare Integrin αvβ3 targeted microbubbles,test physical-chemical properties and targeting study in vitro.Methods Integrin αvβ3 targeted microbubbles (MBIntegrin αvβ3) with FITC labled iRGD peptide were prapared by sh-maleic imide connection method.Then control microbubbles (MBcontrol) was prapared.Physical and chemical properties were test,fluorescent were displayed under fluorescent microscope and the contents of FITC fluorescence by flow cytometry technique of 2 groups of microbubbles were detected.The affinity of MBIntegrin αvβ3 and MBcontrol for bEnd.3 cells was investigated with light and fluorescent microscope.Results ①The particle size of MBIntegrin αvβ3 was (0.84±0.26)μm,and had no statistically significant difference compared with MBcontrol (P>0.05).②Under fluorescent microscope,MBcontrol displayed without fluorescent,while MBIntegrin αvβ3 showed clear green light.③The contents of FITC of MBIntegrin αvβ3was 98.4%,however,the contents of FITC of MBcontrol was 2.5%.④It showed that MBIntegrin αvβ3 adhered to bEnd.3 cells in vitro experiment.Conclusion Integrin αvβ3 targeted microbubbles with iRGD is successfully prepared and proves good targeting ability in vitro. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
