| 钟渝,刘政.微泡增强的超声空化阻断肿瘤微循环的病理观察[J].中国医学影像技术,2015,31(8):1131~1134 |
| 微泡增强的超声空化阻断肿瘤微循环的病理观察 |
| Pathological changes of tumor microcirculation obstruction by microbubbles mediated ultrasound cavitation |
| 投稿时间：2014-09-10 修订日期：2015-05-29 |
| DOI：10.13929/j.1003-3289.2015.08.001 |
| 中文关键词: 微泡 空化 超声学 新生血管化,病理性 动物实验 |
| 英文关键词:Microbubbles Cavitation Ultrasonics Neovascularization, pathologic Animal experimentation |
| 基金项目:重庆市基础与前沿计划项目(cstc2013jcyjA0913)、成都军区医学科学技术研究计划项目面上项目(C14022)。 |
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| 中文摘要: |
| 目的 探讨微泡增强的超声空化阻断肿瘤微循环的病理机制。方法 建立兔肝VX2转移瘤模型18只,随机分为超声微泡组(n=6)、单纯超声组(n=6)及假照组(n=6)。对超声微泡组经耳缘静脉注射微泡,并进行超声辐照;对单纯超声组以生理盐水代替微泡,并进行超声辐照;对假照组注射生理盐水,并进行超声假照。观察辐照后各组病理变化。结果 大体解剖观察,超声微泡组辐照面可见较多出血点;单纯超声组及假照组辐照后肿瘤组织均未见明显改变。光镜下观察,超声微泡组肿瘤组织可见大片出血,血管内皮细胞损伤、连续性中断,红细胞外溢;单纯超声组可见局灶性小片状出血,血管内皮尚完整;假照组肿瘤组织未见明显出血,血管内皮完整。电镜下观察,超声微泡组肿瘤血管内皮破损严重,可见大量红细胞渗出,线粒体肿胀及吞饮小泡;单纯超声组及假照组仅见内质网略水肿。结论 微泡增强的超声空化阻断兔VX2肝脏肿瘤微循环的机制可能为血管机械性损伤。 |
| 英文摘要: |
| Objective To explore the pathological mechanism of microbubbles mediated ultrasound cavitation obstructed tumor microcirculation. Methods Totally 18 New Zealand rabbits bearing liver VX2 tumor were randomly divided into 3 groups (each n=6). The microbubbles injection and ultrasound irradiation were performed in mirobubbles group (US＋MB group). The normal saline injection and ultrasound irradiation were performed in simple ultrasound group (US group). And the normal saline injection and sham ultrasonic irradiation were performed in sham-irradiated group. The pathological changes were observed. Results The gross anatomy showed significant hemorrhage of target region in US＋MB group, while no significant changes in US and sham-irradiated groups. Pathological observation with light microscope showed significant hemorrhage, erythrocyte spillover, vascular endothelial interrupt and cells injured in US＋MB group; few hemorrhage and mild injury of endothelium in US group; unbroken endothelium and insignificant hemorrhage in sham-irradiated group. Pathological observation with electronic microscope showed badly broken endothelium, significant hemorrhage; swelling of mitochondrion and pinocytosis vesicles in US＋MB group, only edema of endoplasmic reticulum in US and sham-irradiated groups. Conclusion The pathological mechanism of microbubbles mediated ultrasound cavitation on tumor microcirculation obstruction maybe vascular mechanical damage. |
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