| 李守强,田家玮,曲绍辉,刘月,付昕,冷晓萍.自制纳米级E-选择素靶向超声微泡对缺血心肌分子成像[J].中国医学影像技术,2015,31(6):803~806 |
| 自制纳米级E-选择素靶向超声微泡对缺血心肌分子成像 |
| Ultrasounic molecular imaging for myocardial ischemic using E-selectin targeting nanometer microbubbles |
| 投稿时间：2015-01-08 修订日期：2015-04-11 |
| DOI：10.13929/j.1003-3289.2015.06.001 |
| 中文关键词: 纳米级微泡 造影剂 超声检查 分子成像 E-选择素 心肌缺血 |
| 英文关键词:Nanometer microbubbles Contrast media Ultrasonography Molecular imaging E-selectin Myocardial ischemia |
| 基金项目:国家自然科学基金(81371569)、黑龙江省教育厅海外学人科研资助项目(1252HQ015)、哈尔滨市科技创新人才研究专项基金项目(2012RFLXS018)。 |
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| 中文摘要: |
| 目的 采用自制的携E-选择素抗体的纳米级靶向超声造影剂对大鼠缺血再灌注损伤后的缺血心肌进行超声分子成像,评估其检测缺血心肌的可行性。方法 采用生物素-链亲和素方法制备纳米级靶向微泡,应用荧光显微镜及流式细胞仪检测微泡形态及抗体连接效率;将26只SD大鼠随机分为E-选择素靶向微泡组(MBE组,n=10)、IGg抗体微泡组(MBIGg组,n=10)、普通微泡组(MBC组,n=6),制备心肌缺血再灌注损伤模型,将冠状动脉左前降支阻断15 min后恢复灌注,于再灌注后6 h经尾静脉分别注射3种微泡行CEUS。后行病理学检查。结果 MBE组缺血区域声学强度(VI)显著高于非缺血区(P<0.05),也显著高于MBC组、MBIGg组缺血区域的VI(P<0.05);MBC组、MBIGg组缺血区域与非缺血区的VI差异无统计学意义(P>0.05)。结论 纳米级E-选择素靶向超声微泡可以早期检测到缺血心肌,有望实现缺血心肌的"记忆"成像。 |
| 英文摘要: |
| Objective To develop an echocardiographic molecular imaging approach for detecting recent myocardial ischemia using self-made E-selectin targeting nanometer microbubbles, and assess the recent cardiac ischemia. Methods Lipid nanometer microbubbles bearing recombinant E-selectin (MBE) or nonspecific IGg (MBIGg) were prepared using avidin-biotin bridge, then observed and evaluated by fluorescence microscope and flow cytometry. Twenty-six rats were randomly divided into MBE, MBIGg, and MBC group. Ischemia-reperfusion injury of myocardium was performed with left anterior descending (LAD) occluded for 15 min and then reperfused, and CEUS with MBE, MBIGg, and MBC was performed 6 h after reperfusion respectively. Postmortem the heart was excised for pathological observation. Results Ultrasound imaging after injection of MBE demonstrated persistent contrast enhancement of the previously ischemic regions. Videointensity (VI) in the postischemic myocardium after injection of MBE was higher than that in nonischemic regions (P<0.05), and higher than that after MBIGg or MBC injection (all P<0.05). VI in the postischemic regions after MBIGg or MBC injection was not significantly different from the nonischemic regions (P>0.05). Conclusion Echocardiographic molecular imaging with nanometer microbubbles bearing E-selectin antibodies could detect recent ischemia, setting the stage for myocardial ischemic memory imaging. |
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