| 徐芬芬,王志刚,李攀,郝兰,曹阳,王冬.载10-羟基喜树碱液态氟碳纳米粒的声致相变条件及释药性能[J].中国医学影像技术,2015,31(2):196~200 |
| 载10-羟基喜树碱液态氟碳纳米粒的声致相变条件及释药性能 |
| Acoustic phase transition conditions of 10-HCPT loaded perfluoropentane nanoparticles and its drug release prorperties |
| 投稿时间：2014-08-27 修订日期：2014-11-18 |
| DOI：10.13929/j.1003-3289.2015.02.011 |
| 中文关键词: 超声学 载药纳米粒 声致相变 释药 细胞毒性 |
| 英文关键词:Ultrsonics 10-HCPT loaded nanoparticles Acoustic droplet vaporization Drug release Cell toxicity |
| 基金项目:国家自然科学基金面上项目(81371578)、重庆高校创新团队建设计划(KJTD201303)、国家自然科学基金重点项目(81130025)、国家自然科学基金"2011中加(NSFC-CIHR)健康研究合作计划项目"(81161120548)、靶向纳米微泡联合高强度聚焦超声消融乳腺肿瘤的应用及基础研究项目(81371579) |
| 作者 | 单位 | E-mail | | 徐芬芬 | 重庆医科大学超声影像学研究所, 重庆 400010
重庆医科大学附属儿童医院超声科, 重庆 400010 | | | 王志刚 | 重庆医科大学超声影像学研究所, 重庆 400010
重庆医科大学附属儿童医院超声科, 重庆 400010 | | | 李攀 | 重庆医科大学超声影像学研究所, 重庆 400010
重庆医科大学附属儿童医院超声科, 重庆 400010 | cqlipan@163.com | | 郝兰 | 重庆医科大学超声影像学研究所, 重庆 400010
重庆医科大学附属儿童医院超声科, 重庆 400010 | | | 曹阳 | 重庆医科大学超声影像学研究所, 重庆 400010
重庆医科大学附属儿童医院超声科, 重庆 400010 | | | 王冬 | 重庆医科大学超声影像学研究所, 重庆 400010
重庆医科大学附属儿童医院超声科, 重庆 400010 | |
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| 中文摘要: |
| 目的 探讨载10-羟基喜树碱液态氟碳纳米粒的声致相变条件、增强超声显像效果和纳米粒释药特性及对肝癌细胞株(SMMC-7721)的生长抑制作用。方法 应用低强度聚焦超声(LIFU)辐照载药液态氟碳纳米粒溶液,观察纳米粒显像的最佳功率及最佳浓度,紫外分光光度计测定不同药脂比纳米粒的药物包封率,透析法观察10-HCPT的释放,CCK-8试剂检测载药液态氟碳纳米粒对肝癌细胞株(SMMC-7721)的细胞毒性作用。结果 在体外经LIFU辐照后,纳米粒的最佳显像辐照功率为5 W、时间为2 min,最佳浓度为8.53×109/ml,当药脂比为1:10时,其包封率最高为(87.03±2.55)%,超声作用后载药纳米粒缓慢释放,载药纳米粒在48 h后能显著抑制肝癌细胞株(SMMC-7721)的生长。结论 载10-羟基喜树碱液态氟碳纳米粒在合适的条件下可发生声致相变,显著增强超声显像,并能同时缓慢释放药物从而抑制肝癌细胞株(SMMC-7721)的生长。 |
| 英文摘要: |
| Objective To study the acoustic phase transition conditions of 10-HCPT loaded perfluoropentane nanoparticles and their enhancement effect for ultrasonic imaging, and observe their drug release prorperties and cell toxicity on human hepatoma SMMC-7721 in vitro. Methods The emulsions of drug-loaded nanoparticles were irradiated by low intensity focused ultrasound (LIFU). The power of LIFU and the concentration of nanoparticles for imaging were studied in order to acquire the best visualization. The entrapment efficiency with different mass ratio of drug and phospholipid was determined with ultraviolet spectrophotometer. The release of 10-HCPT was observed with dialysis method. The cytotoxic effect of drug-loaded nanoparticles on human hepatoma SMMC-7721 was measured by cell counting kit-8 (CCK-8). Results After being irradiated by LIFU, the best irradiation power was 5 W, the irradiation time was 2 min and the concentration of nanoparticles was 8.53×109/ml. The highest entrapment efficiency ([87.03±2.55]%) was obtained when the mass ratio of drug and phospholipid was 1:10. The drug was released slowly from the drug-loaded nanoparticles and significantly inhibited the proliferation of human hepatoma SMMC-7721 after 48 h. Conclusion As phase-shift and 10-HCPT loaded perfluoropentane nanoparticles, it can enhance ultrasound imaging greatly with appropriate conditions, which can lead to releasing drug slowly to inhibit the proliferation of human hepatoma SMMC-7721. |
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