| 张斌,冉海涛,王志刚,苏蕾,郝兰."适配子-PLGA纳米粒"靶向超声/光声相变造影剂的制备[J].中国医学影像技术,2014,30(11):1609~1613 |
| "适配子-PLGA纳米粒"靶向超声/光声相变造影剂的制备 |
| Preparation of "aptamer-PLGA nanoparticle" as targeted, phase-transition based ultrasound/photoacoustic dual-modality imaging contrast agent |
| 投稿时间：2014-07-03 修订日期：2014-09-17 |
| DOI： |
| 中文关键词: 造影剂 适配子 相变 纳米技术 |
| 英文关键词:Contrast Media Aptamer Phase transition Nanotechnology |
| 基金项目: |
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| 中文摘要: |
| 目的 研制一种适配子偶联的包裹纳米金棒和液态氟碳的PLGA纳米粒。方法 采用双乳化法制备包裹纳米金棒及全氟己烷(PFH)的PLGA纳米粒(GNP);用碳二亚胺法将适配子与PLGA纳米粒连接获得"适配子-PLGA纳米粒"靶向相变造影剂(AP-GNP)。光镜、激光共聚焦显微镜及电镜下观察及检测AP-GNP基本性质;激光共聚焦显微镜下及流式细胞术观察及检测适配子与纳米粒连接情况;以激光仪辐照AP-GNP观察其光致相变情况;观察AP-GNP与乳腺癌细胞MCF-7特异性结合情况。结果 成功制备适配子修饰的包裹纳米金棒和PFH的PLGA纳米粒, 其平均粒径为(472.43±25.82)nm, 适配子与纳米粒的连接率为达97.98%;激光辐照AP-GNP后, 光致相变效果明显;AP-GNP粘附于MCF-7细胞周围, 未修饰适配子GNP未见明显特异性结合。结论 成功制备偶联的包裹纳米金棒和PFH的PLGA纳米粒, 其光致相变效果明显, 靶向性能良好。 |
| 英文摘要: |
| Objective To synthesize an aptamer-labeled ultrasound/photoacoustic dual-modality imaging contrast agent loaded with gold nanorods and liquid perflurocarbon. Methods The aptamer-labeled PLGA nanoparticle contrast agent was loaded with gold nanorod and liquid perflurocarbon. The nanoparticle (GNP) was synthesized via double emulsion technique and the conjugation with aptamer (AP-GNP) was achieved with carbodiimide technique. The basic properties of AP-GNP were observed and measured under light microscope, laser confocal microscopy and electron microscope. The conjugation of AP-GNP was observed and measured using laser confocal microscopy and flow cytometry. AP-GNP were irradiated with nanosecond pulsed laser to investigate its phase-transition property. The specific binding of AP-GNP with the breast cells MCF-7 was observed. Results The aptamer-labeled ultrasound/photoacoustic dual-modality imaging contrast agent were successfully prepared with a mean diameter of (472.43±25.82)nm and binding efficiency of 97.98% binding efficiency between aptamer and nanoparticle. After irradiation with laser, significant phase-transition phenomenon of AP-GNP was observed. AP-GNP were conjugated and crowded around the MCF-7 cells, however the GNP were fail to conjugate. Conclusion The aptamer-labeled ultrasound/photoacoustic dual-modality imaging contrast agent loaded with gold nanorods and liquid perflurocarbon are successfully prepared with excellent phase-transition and target property. |
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