| 鲁琳,娄明武,申云霞,梁冰,颜妙如,钟欢欢.猪冠状动脉微栓塞致心肌梗死模型的建立[J].中国医学影像技术,2014,30(11):1605~1608 |
| 猪冠状动脉微栓塞致心肌梗死模型的建立 |
| Myocardial infarction animal model induced by percutaneousendovascular microembolism in pig model |
| 投稿时间：2014-07-02 修订日期：2014-08-21 |
| DOI： |
| 中文关键词: 体层摄影术,X线计算机 心肌梗死 冠状动脉微栓塞 模型,动物 猪 |
| 英文关键词:Tomography, X-ray computed Myocardial infarction Coronary microembolization Models, animal Swine |
| 基金项目:中国博士后科学基金(2012M511785)、广东省自然科学基金(2012M511785)、深圳市龙岗区科技计划医疗卫生项目(YS2013096)、深圳市龙岗区"专家提升计划"资助项目。 |
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| 中文摘要: |
| 目的 以聚乙烯醇(PVA)为栓塞剂, 采用经皮微导管介入方法建立可长期存活的猪冠状动脉微栓塞(CME)致心肌梗死模型。方法 对16只西藏小型猪经股动脉选择性插管, 于左前降支动脉第一对角支开口处远端缓慢注入不同浓度100 μm PVA, 于术后第3天、第60天行640层容积CT心肌灌注成像;动物处死后对心肌组织行TTC染色和病理学观察。结果 9只动物造模成功并长期存活, 其PVA浓度为0.001 ml/ml, 注射总量10 ml;CT心肌灌注成像示左心室心尖前壁、侧壁及间隔壁灌注缺损, 主要位于心内膜下, 部分累及心外膜下心肌;心脏TTC染色和病理示微血管栓塞区域心肌梗死。结论 以PVA颗粒为栓子, 采用经皮微导管介入方法建立猪CME致心肌梗死模型成功率高、存活时间长, 为临床研究CME致心肌梗死的理想动物模型。 |
| 英文摘要: |
| Objective To establish a myocardial infarction animal model induced by coronary microembolization (CME) with long-term surviving using percutaneous endovascular technique with polyvinyl alcohol (PVA) as emboliaztion agents in pigs. Methods CME was induced by selective infusion different doses of PVA microspheres (100 μm) into the distal of first diagonal branch of left anterior descending artery in 16 Tibet pigs. CT myocardial perfusion imaging (MPI) using 640-slice volumetric CT scanning were performed in the 3rd and 60th day after operation. Myocardial infarction was demonstrated using TTC staining and pathology observation after the animals were sacrificed. Results The model were successfully established with PVA concentration of 0.001 ml/ml and dose of 10 ml in 9 pigs with long-term survival. CT MPI showed perfusion defects in apical anterior wall, lateral wall and septal wall of the left ventricular, which mainly involved subendocardial, partly involved epicardial myocardium. TTC staining and pathology demonstrated myocardial infarction in microembolization region. Conclusion Estabishment of myocardial infarction animal model induced by CME using percutaneous endovascular technique with 100 μm PVA as emboliaztion agents in pigs has high success rate and the animal has long-term survival time. It is an ideal animal model for investigating mechanism of myocardial infarction induced by CME. |
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