| 吴欢,许晓琳,智慧,阮镜良,杨海云,肖晓云,罗葆明.声动力学治疗对MDA-MB-231细胞增殖及侵袭能力的影响[J].中国医学影像技术,2014,30(2):180~184 |
| 声动力学治疗对MDA-MB-231细胞增殖及侵袭能力的影响 |
| Impact of sonodynamic therapy on proliferation and invasive ability of MDA-MB-231 cell line |
| 投稿时间：2013-09-03 修订日期：2014-01-01 |
| DOI： |
| 中文关键词: 血卟啉单甲醚 声动力学治疗 侵袭性 |
| 英文关键词:Hematoporphyrin monomethyl ether Sonodynamic therapy Invasive ability |
| 基金项目:广东省医学科学技术研究基金（B2010085、A2013205）；教育部博士点基金（博导类）（20120171110080）。 |
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| 中文摘要: |
| 目的 探讨超声辐照血卟啉单甲醚和声诺维对乳腺癌细胞株MDA-MB-231增殖及侵袭能力的影响。方法 将对数生长期的MDA-MB-231细胞制成细胞悬液，加入HMME和SonoVue后用超声进行辐照。分3组：对照组，不接受治疗；辐照组1：输出声强0.3 W/cm2，辐照时间60 s；辐照组2：输出声强0.6 W/cm2，辐照时间30 s。采用MTS法绘制细胞生长曲线，平板克隆方法观察细胞形成克隆的能力，并用Transwell小室法检测细胞侵袭能力。结果 对照组的生长曲线陡直，而辐照组的曲线平缓。接种2周后对照组出现肉眼可见的细胞克隆，而辐照组未出现。结晶紫染色后观察，辐照组仅可见由少许细胞聚集成的细胞团。接种后24 h、48 h观察，对照组、辐照组1、辐照组2穿越小室的细胞数分别为42.00±13.52、3.52±1.26、2.75±0.96（P<0.05）及104.25±12.61、36.25±3.40、28.75±4.99（P<0.05）。对照组穿越小室的细胞数显著高于辐照组（P<0.05）。结论 超声辐照联合HMME及SonoVue能有效抑制MB-231细胞增殖，降低细胞形成克隆的能力，并显著降低细胞的侵袭能力。 |
| 英文摘要: |
| Objective To investigate the impact of synergy of hematoporphyrin monomethyl ether (HMME) and SonoVue under ultrasound irradiation on proliferation and invasive ability of MDA-MB-231 cell line. Methods Cell suspension of MDA-MB-231 cells in logarithmic growth phase, mixed with HMME and SonoVue underwent ultrasound irradiation. There were 3 groups, i.e. control group (without irradiation), irradiation group 1 (sound intensity 0.3 W/cm2, irradiation time 60 s) and irradiation group 2 (sound intensity 0.6 W/cm2, irradiation time 30 s). Growth curve obtained by MTS method was drawn, plate clone formation assay was performed, and changes of invasive ability after irradiation with Transwell assay were detected. Results Irradiation groups showed a flat curve, while control group had a sharp rise curve. Cell clones visible to naked eyes emerged 2 weeks after inoculation in control group, but not in irradiation groups. After crystal violet staining, only cell clusters composed of small amount of cells were seen in irradiation groups. The number of cells transited through matrigel were 42.00±13.52, 3.52±1.26, 2.75±0.96 in control group, irradiation group 1 and irradiation group 2 24 h after inoculation, and 104.25±12.61, 36.25±3.40, 28.75±4.99 48 h after inoculation, respectively (all P<0.05).The number of cells transited through matrigel of control group were higher than that of irradiation groups (P<0.05). Conclusion Synergy of HMME and SonoVue under ultrasound irradiation could inhibit proliferation of MB-231 cells, reduce cell clone formation and attenuated cell invasive ability effectively and significantly. |
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