| 谢佩怡,全力,任杰,张英才,姜楠,张建生,孟晓春,朱康顺,单鸿.应用四维CT灌注技术分析肝切除术后大鼠残肝微循环血流动力学变化[J].中国医学影像技术,2014,30(2):171~175 |
| 应用四维CT灌注技术分析肝切除术后大鼠残肝微循环血流动力学变化 |
| Analysis of hemodynamic changes of microcirculation after different partial hepatectomy for rat models with 4D-CT perfusion imaging |
| 投稿时间：2013-04-24 修订日期：2013-08-03 |
| DOI： |
| 中文关键词: 肝切除术 体层摄影术,X线计算机 血流动力学 灌注成像 |
| 英文关键词:Hepatectomy Tomography, X-ray computed Hemodynamics Perfusion imaging |
| 基金项目:国家自然科学基金（81201090、30901465）；广东省自然科学基金（S2012010008367）；教育部第43批留学回国人员科研启动基金；广东省科技计划项目（2010B031600053）；高校基本业务费青年教师培育项目（12ykpy47）。 |
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| 中文摘要: |
| 目的 应用四维CT灌注（4D-CTP）技术研究不同比例肝切除（PH）术后大鼠残肝微循环血流动力学变化。方法 将144只SD大鼠随机分入假手术（sham）组及50%、60%、70%、80% PH组，每组24只，余24只做补充实验。术后1 h、1、3和7天，sham组和手术组各取6只大鼠行4D-CTP检查，计算肝动脉灌注量（HAP）、门静脉灌注量（PVP）、总肝灌注量（TLP）和肝动脉灌注指数（HPI），之后取肝脏行病理学检查。结果 共21只大鼠符合小肝综合征（SFSS）表现，70% PH组1只，80% PH组20只。各PH组PVP、TLP均于术后1 h达峰值，术后1天下降，术后3、7天趋于稳定；50%、60% PH组HAP、HPI术后1 h、1天降低，3天和7天略增高；70%、80% PH组HAP术后1 h最高，术后1、3、7天逐渐下降，HPI于术后1 h显著降低，术后1天进一步轻度降低，术后3、7天稍增高。术后1 h，80% PH组PVP、TLP高于50%、60% PH组和sham组（P均<0.05），HPI低于sham组（P<0.05）；术后1天，80% PH组PVP、TLP高于sham组（P均<0.05），HPI低于sham组（P<0.05）；术后3、7天各组PVP、TLP、HPI、HAP 差异均无统计学意义（P均>0.05）。结论 应用4D-CTP技术可定量分析大鼠PH术后残肝血流动力学变化，有效区分肝动脉、门静脉血流灌注异常。 |
| 英文摘要: |
| Objective To observe microcirculatory hemodynamic changes of remnant liver after partial hepatectomy (PH) with different proportions for rat models using four-dimensional CT perfusion (4D-CTP) technique. Methods A total of 144 SD rats were randomly divided into sham group, 50%, 60%, 70%, 80% PH group and supplemental group (n=24). At 1 h, 1 day, 3 days and 7 days after PH operation, 4D-CTP scan was administrated for 6 rats from each group. Hepatic artery perfusion (HAP), portal vein perfusion (PVP), total liver perfusion (TLP) and hepatic perfusion index (HPI) were calculated based on 4D-CTP raw data. Then the remnant liver was taken for histopathological analysis. Results Small-for-size syndrome (SFSS) was found in 21 rats, 1 in 70% PH group and 20 in 80% PH group. In all PH groups, PVP and TLP increased and reached to the peak value 1 h after operation, descended 1 day after operation, stabilized 3 and 7 days after operation. In 50% and 60% PH group, HPI and HAP descended 1 h and 1 day after operation, increased 3 and 7 days after operation. In 70% and 80% PH group, HAP increased and reached to the peak value 1 h after operation, descended 1, 3 and 7 days after operation, HPI descended 1 h and 1 day after operation, increased 3 and 7 days after operation. At 1 h after operation, in 80% PH group, PVP and TLP were significantly higher than those in 50% and 60% PH groups, as well as in sham group (all P<0.05), HPI was significantly lower than that in sham group (P<0.05). One day after operation, PVP and TLP in 80% PH group were still higher than those in sham group (all P<0.05), HPI was significantly lower than that in sham group (P<0.05). Three and 7 days after operation, there was no significant difference of PVP, TLP, HAP, HPI among all groups (all P>0.05). Conclusion Using 4D-CTP, hemodynamic changes of microcirculatory for remnant liver rat after PH can be obtained quantatively, which can effectively distinguish hepatic artery and portal vein perfusion. |
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