| 韩海伟,韩成坤,庄碧梅,张惠杰,庄雄杰.3.0T 1H-MRS测定大鼠肝内脂肪含量的可行性[J].中国医学影像技术,2014,30(2):167~170 |
| 3.0T 1H-MRS测定大鼠肝内脂肪含量的可行性 |
| Assessment of hepatic lipid in steatoic liver rat models using 1H-MRS on 3.0T |
| 投稿时间：2013-09-06 修订日期：2013-12-04 |
| DOI： |
| 中文关键词: 脂肪肝 磁共振波谱 脂质含量 |
| 英文关键词:Fatty liver Magnetic resonance spectroscopy Content of lipid |
| 基金项目: |
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| 中文摘要: |
| 目的 探讨利用3.0T临床型MR仪行1H-MRS测定非酒精性脂肪肝（NAFLD）大鼠模型肝内脂肪含量的可行性。方法 将75只SD大鼠随机分为高脂饲养实验组（n=50）与正常对照组（n=25）；分别在第2、4、6、8、10周时行肝脏1H-MRS扫描及组织病理学检查。将1H-MRS原始数据导入LC model软件进行分析，并对各代谢物浓度行T1、T2纠正；将1H-MRS结果与组织病理学结果加以对照，将肝内饱和脂肪酸含量、总非不饱和脂肪酸含量及组织病理学检测含量分别记为LipMRS1、LipMRS2和FI。结果 大鼠肝脏同一部位相同参数（r1）及同一部位不同时间点（r2）1H-MRS测量结果（r1=0.99，r2=0.96，P均<0.01）呈高度正相关；LipMRS1、FI呈高度正相关，回归方程为：LogY=0.37+1.21LogX（r=0.95，P<0.01），NAFLD大鼠模型中LipMRS1及LipMRS2含量无明显相关性（r=－0.11，P=0.66）；Bland & Altman曲线分析显示LipMRS1与FI的一致性好。结论 采用3.0T临床型MR仪行1H-MRS可作为在体评价NAFLD大鼠模型肝内脂肪含量的无创的影像学方法；LC model软件能区分肝内脂肪组分并精确定量。 |
| 英文摘要: |
| Objective To investigate the feasibility of in vivo assessment of hepatic lipid content using clinical 3.0T 1H-MRS in a nonalcoholic fatty liver disease (NAFLD) rat models. Methods Totally 75 healthy Sprague-Dawle rats were randomly divided into NAFLD group (n=50) and control group (n=25). 1H-MRS of the liver was performed, and then rats were sacrificed for histopathological assessment of the liver after 2, 4, 6, 8, 10 weeks. The raw data of 1H-MRS was exported to LC model software. The metabolite concentration of H2O and Lipid was measured and corrected for T1 and T2 decay. The ratio of Lip/(Lipcorr+H2Ocorr)×100% was noted as LipMRS1, of unsaturated fatty acid (UFA)/(UFA+H2O)×100% was noted as LipMRS2, the lipid content estimated by histopathology was noted as FI. Results The reproducibility of 1H-MRS was validated by showing that duplicate 1H-MRS measurements on different occasions (r1) and different sites (r2) of the liver were highly correlated (r1=0.99, r2=0.96, all P<0.01). LipMRS1 and FI were high positively correlated, and the regression equation was LogY=0.37+1.21LogX (r=0.95, P<0.01), while LipMRS1 and LipMRS2 had no correlation (r=-0.11, P=0.66). With Bland-Altman method plot, all data points of LipMRS1 and FI were within the limits of agreement. Conclusion High quality and reproducible 1H-MRS of NAFLD rat model can be achieved using clinical 3.0T MR scanner. The saturated fatty acid and the polyunsaturated fatty acid can be discriminated by LC model software. 1H-MRS can be used as a noninvasive and in vivo method in assessing hepatic lipid content and composition for NAFLD rat models. |
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