| 张苗苗,章杰,徐志峰,陈耀文,陈义兵,吴仁华.9.4T 1H-MRS定量分析三种乳腺癌细胞的水溶性代谢物[J].中国医学影像技术,2013,29(6):890~893 |
| 9.4T 1H-MRS定量分析三种乳腺癌细胞的水溶性代谢物 |
| Quantitative analysis on water-soluble metabolites of three kinds of breast carcinoma cells with 9.4T 1H-MRS |
| 投稿时间：2012-09-17 修订日期：2013-05-15 |
| DOI： |
| 中文关键词: 磁共振波谱 乳腺肿瘤 代谢物 细胞 |
| 英文关键词:Magnetic resonance spectroscopy Breast neoplasms Metabolite Cells |
| 基金项目:国家自然科学基金重点项目(30930027);国家自然科学基金(60971075);广东省自然科学基金(S2011010005194);汕头大学科研启动基金(NTF10010)。 |
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| 中文摘要: |
| 目的 利用9.4T磁共振频谱仪离体检测3种乳腺癌细胞的水溶性代谢物频谱特征。 方法 培养获取ZR-75-1、T47-D及SK-BR-3乳腺癌细胞,用高氯酸提取水溶性代谢物,以9.4T高场磁共振仪检测所提取水溶性代谢物的氢质子频谱,定量计算谱线中相应代谢物的浓度并分析其特征。 结果 ZR-75-1、T47-D及SK-BR-3乳腺癌细胞具有3种不同代谢物特征;3种乳腺癌细胞在乳酸及胆碱处均出现波峰,且T47-D的乳酸及胆碱浓度高于ZR-75-1和SK-BR-3。 结论 利用9.4T磁共振频谱仪能获取3种乳腺癌细胞的代谢信息;T47-D乳腺癌细胞内乳酸及胆碱代谢高于ZR-75-1和SK-BR-3。 |
| 英文摘要: |
| Objective To explore the features of spectrum of water-soluble metabolites of 3 kinds of breast cancer cells in vitro with 9.4T 1H-MRS. Methods Three kinds of human breast carcinoma cells (ZR-75-1, T47-D, SK-BR-3) were cultured and collected. The metabolites were extracted with perchloric acid and prepared for MRS analysis. 1H spectra were acquired with 9.4T high resolution MRS, and the concentrations of main metabolites were quantitatively calculated. Results ZR-75-1, T47-D and SK-BR-3 breast cancer cells had characteristics of three different metabolites. The metabolic peak of lactate and choline were observed in all 3 kinds of breast cancer cells. The concentration of choline-compounds and lactate in T47-D were higher than those of ZR-75-1 and SK-BR-3. Conclusion The metabolic profiles of three subtypes of human breast carcinoma cells can be reliably detected and quantified with 9.4T MRS. The metabolic of lactate and choline-compounds of T47-D cell are higher than those of ZR-75-1 and SK-BR-3. |
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