| 韩雪,程文,郑建,荆慧,邵华.超声联合SonoVue微泡介导NET-1siRNA转染人肝癌细胞[J].中国医学影像技术,2012,28(8):1465~1469 |
| 超声联合SonoVue微泡介导NET-1siRNA转染人肝癌细胞 |
| NET-1 siRNA transfected into HepG2 cells by SonoVue microbubbles combined with ultrasound |
| 投稿时间：2012-03-05 修订日期：2012-04-12 |
| DOI： |
| 中文关键词: 超声检查 微泡 RNA干扰 肝肿瘤 |
| 英文关键词:Ultrasonography Microbubbles RNA interference Liver neoplasms |
| 基金项目:黑龙江省自然科学基金(D201170)。 |
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| 摘要点击次数: 3064 |
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| 中文摘要: |
| 目的 探讨超声联合微泡造影剂介导NET-1 siRNA在人肝癌细胞中的转染效果。方法 将培养的HepG2细胞分为5组:A组为空白对照;B组培养瓶中仅加入NET-1 siRNA;C组为加入脂质体及NET-1 siRNA;D组为加入造影剂及NET-1 siRNA并给予超声辐照;E组为加入脂质体、造影剂、NET-1 siRNA,并给予超声辐照。之后以荧光显微镜检测NET-1 siRNA转染率,RT-PCR法检测各组细胞NET-1 mRNA的基因表达,Western-Blotting法检测各组细胞NET-1表达情况,MTT检测HepG2细胞生长情况。结果 与C组、D组相比,E组NET-1 siRNA转染率明显提高(P<0.05);D组和E组细胞内NET-1 mRNA及NET-1蛋白的表达抑制率均高于其他各组(P均<0.01),而转染后24~72 h HepG2细胞增殖受到明显抑制。结论 超声联合微泡造影剂能有效促进NET-1 siRNA在肝癌细胞中的表达,并抑制肝癌细胞的增殖。 |
| 英文摘要: |
| Objective To investigate the effect of NET-1 siRNA transfected into HepG2 cells by microbubbles contrast agents combined with ultrasound exposure. Methods HepG2 cells were divided into 5 groups, i.e. control group (Group A), NET-1 siRNA group (Group B), Lipofectamine+NET-1 siRNA group (Group C), SonoVue+NET-1 siRNA group (Group D) and Lipofectamine+NET-1 siRNA+SonoVue group (Group E). The latter two groups were irradiated by ultrasound. Then the transfection rates of siRNA were observed using fluorescence microscope. RT-PCR and Western-Blotting were used to evaluate the expression of NET-1 mRNA and NET-1 protein. The proliferation of HepG2 cells was observed by MTT assay. Results The NET-1 siRNA transfection rate of Group E was higher than that of Group C and Grops D (P<0.05), the protein expression inhibition rate of NET-1 mRNA and NET-1 protein of group D and E were higher than those of the other groups (P<0.01), while the proliferation of cells were significantly inhibited 24 to 72 h after transfection. Conclusion The method of ultrasound contrast agents combined with ultrasound exposure can increase the transfection effect of NET-1 siRNA and inhibit the proliferation of HepG2 cells. |
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