| 潘迪,严飞,郑海荣,吴瑞凤,邱本胜.叶酸介导APTMS包被的超小超顺磁性氧化铁的制备及体外MR成像[J].中国医学影像技术,2012,28(5):838~842 |
| 叶酸介导APTMS包被的超小超顺磁性氧化铁的制备及体外MR成像 |
| Preparation and in vitro MR imaging of folic acid-conjuncted APTMS coated ultrasmall superparamagnetic iron oxide |
| 投稿时间：2011-09-02 修订日期：2011-10-10 |
| DOI： |
| 中文关键词: 叶酸 纳米微粒 磁共振成像 |
| 英文关键词:Folic acid Nanoparticles Magnetic resonance imaging |
| 基金项目: |
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| 中文摘要: |
| 目的 探究叶酸介导的超小超顺磁性氧化铁(USPIO)对于人乳腺癌MCF-7细胞表面叶酸受体的靶向性及MR成像的可行性。方法 ①制备叶酸介导的耦联3-氨基丙基-三甲氧基硅烷(APTMS)的USPIO(FA-APTMS-USPIO),通过TEM、FTIR等技术对其进行表征。②USPIO组、竞争抑制组(FA-APTMS-USPIO+叶酸)及叶酸介导的耦联APTMS的靶向组(FA-APTMS-USPIO)分别与MCF-7细胞孵育不同时间,通过普鲁士蓝染色观察不同组别铁颗粒的摄取情况。③对与纳米铁孵育后的细胞采用3.0T MR仪进行体外MR成像。④采用MTT法进行细胞活性检测。结果 透射电镜照片显示FA-APTMS-USPIO外形较规则。普鲁士蓝染色观察,靶向组细胞摄取的氧化铁较多,可见大量蓝色沉淀。竞争抑制组与USPIO组细胞内蓝色颗粒较少。体外MRI结果显示,与MCF-7孵育的靶向组T2信号降低显著,竞争抑制组和USPIO组无明显变化。MTT实验显示,靶向组不同时间对细胞生存能力无显著影响。结论 FA-APTMS-USPIO对MCF-7细胞有良好的靶向性,对早期诊断乳腺癌具有重大应用潜力。 |
| 英文摘要: |
| Objective To investigate the targeting efficiency of folic acid-conjunct APTMS-coated ultrasmall superparamagnetic iron oxide (FA-APTMS-USPIO) to MCF-7 cells via folic acid receptors, and to assess its potential application to MRI. Methods The following methods were used, including: ①Preparation of FA-APTMS-USPIO and characterization with TEM and FTIR. ②Plain USPIO group, FA-APTMS-USPIO group and competitive inhibition group (FA-APTMS-USPIO plus free folate acids) were setup in the experiment to be used to incubate with MCF-7 for 30 min, 1 h and 2 h, respectively, followed by staining with Prussian blue solution and examined under microscope. ③3.0T MR scanner was utilized to detect the signals of cells incubated with USPIO. ④MTT assay was performed to examine the cell viability after USPIO incubation. Results TEM assay indicated the FA-APTMS-USPIO showed a uniform appearance. Much more blue iron oxide particles were observed in FA-APTMS-USPIO group, but just few were found in USPIO group or competitive inhibition group. In addition, MRI detection demonstrated significant decrease of T2 signal intensity for MCF-7 cells incubated with FA-APTMS-USPIO, while in control group and competitive group did not change obviously. MTT assay showed that the cytotoxicity did not obviously change in FA-APTMS-USPIO groups. Conclusion FA-APTMS-USPIO possesses a good targeting capability to MCF-7 cells and has great potential in early diagnose of breast tumor. |
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