| 章雅琴,胡跃群,廖云杰,容鹏飞,王维.犬肝纤维化模型MR弥散张量成像及其与CT灌注参数、VEGF表达的相关性[J].中国医学影像技术,2012,28(2):216~220 |
| 犬肝纤维化模型MR弥散张量成像及其与CT灌注参数、VEGF表达的相关性 |
| Evaluation of MR DTI on hepatic fibrosis in dog models and the correlation with CT perfusion and VEGF expression |
| 投稿时间：2011-09-18 修订日期：2011-10-09 |
| DOI： |
| 中文关键词: 肝硬化 扩散磁共振成像 体层摄影术,X线计算机 灌注 狗 |
| 英文关键词:Liver cirrhosis Diffusion magnetic resonance imaging Tomography X-ray computed Perfusion Dogs |
| 基金项目:国家自然科学基金(30900359)。 |
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| 中文摘要: |
| 目的 利用DTI测定犬肝纤维化模型的ADC值、FA值,评估肝纤维化程度,分析其与CT灌注参数及VEGF表达水平的相关性。方法 健康中华田园犬15只,随机分成实验组(n=10)和对照组(n=5)。实验组犬经腹腔注射50% CCl4油溶液,辅以高脂饮食,建立犬肝纤维化模型。对2组犬每隔4周行MR DTI、CT灌注成像及肝穿活检。根据病理分期分组,分析各组MR DTI参数ADC值、FA值,并与CT灌注参数及VEGF表达水平进行对照。结果 成功获得各期肝纤维化模型;随肝纤维化进展,ADC值逐渐降低(P<0.05),FA值总体呈上升趋势(P>0.05);ADC值与PVP、TLP呈正相关;VEGF表达水平随肝纤维化进展而明显增高(P<0.05)。结论 对犬行腹腔注射CCl4油溶液辅以高脂饮食可成功模拟人类从肝细胞变性-肝纤维化-肝硬化的全过程;MR DTI能反映肝纤维化各期的血流动力学变化趋势;VEGF可能在慢性肝病所致肝纤维化过程中起重要作用。 |
| 英文摘要: |
| Objective To measure the ADC and FA values of liver fibrosis of dog models with DTI, and to analyze the correlation with CT hemodynamic indexes and VEGF expression. Methods Fifteen healthy Chinese rural dogs were divided into experimental group (n=10) and control group (n=5). Liver fibrosis was induced in dogs in experimental group by intraperitoneal injection of CCl4 and fed with high-fat diet. MR DTI scan, CT perfusion scan and liver biopsies were performed every 4 weeks. DTI parameters were measured according to the pathology, and the correlation with CT hemodynamic indexes and VEGF expression were analyzed. Results The dogs in experimental group were successfully induced into different degrees of liver fibrosis. ADC values declined gradually (P<0.05), while FA values increased (P>0.05) with liver fibrosis deteriorated. ADC values were positively correlated with PVP and TLP values. The VEGF expression ascended significantly with liver fibrosis progressed (P<0.05). Conclusion The dog models that simulating human hepatic fibrosis can be induced successfully by CCl4 and high fat diet. DTI can reflect the tendency of hemodynamic in different degrees of fibrosis. VEGF plays an important role in fibrogenesis procession. |
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