| 杨娅,王艳红,谢谨捷,李嵘娟,李治安,王征,宋砾,罗向红,苏瑞娟,秦彦文.载脂蛋白E基因敲除小鼠早期动脉粥样硬化超声生物显微镜成像与组织病理的对照分析[J].中国医学影像技术,2012,28(1):44~47 |
| 载脂蛋白E基因敲除小鼠早期动脉粥样硬化超声生物显微镜成像与组织病理的对照分析 |
| Ultrasound biomicroscopy imaging of atherosclerosis in the early stage of ApoE-/- mice: Comparison with histopathological results |
| 投稿时间：2011-07-19 修订日期：2011-09-01 |
| DOI： |
| 中文关键词: 超声生物显微镜 动脉硬化 小鼠 载脂蛋白E 病理学 |
| 英文关键词:Ultrasound biomicroscopy Atherosclerosis Mice Apolipoprotein E Pathology |
| 基金项目:国家自然科学基金(30672000、81171351)、北京市自然科学基金(7102044、7082020)。 |
| 作者 | 单位 | E-mail | | 杨娅 | 首都医科大学附属北京安贞医院超声科,北京 100029 | yangya99@hotmail.com | | 王艳红 | 首都医科大学附属北京安贞医院超声科,北京 100029 | | | 谢谨捷 | 首都医科大学附属北京安贞医院超声科,北京 100029 | | | 李嵘娟 | 首都医科大学附属北京安贞医院超声科,北京 100029 | | | 李治安 | 首都医科大学附属北京安贞医院超声科,北京 100029 | | | 王征 | 首都医科大学附属北京安贞医院超声科,北京 100029 | | | 宋砾 | 首都医科大学附属北京安贞医院超声科,北京 100029 | | | 罗向红 | 首都医科大学附属北京安贞医院超声科,北京 100029 | | | 苏瑞娟 | 首都医科大学附属北京安贞医院超声科,北京 100029 | | | 秦彦文 | 首都医科大学附属北京安贞医院超声科,北京 100029 | |
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| 中文摘要: |
| 目的 利用超声生物显微镜(UBM)成像分析载脂蛋白E基因敲除(ApoE-/-)小鼠主动脉早期动脉粥样硬化的超声特征。方法 以8周及16周龄雄性ApoE-/-小鼠各8只为实验组,8周及16周龄雄性C57BL/6小鼠为对照组,用UBM观察小鼠主动脉根部、升主动脉和主动脉弓的形态结构,并于主动脉根部短轴切面测量内-中膜厚度(IMT),与相对应血管节段的病理组织学测值进行对比。结果 ApoE-/-小鼠的主动脉根部IMT较同周龄对照组增厚(P<0.01);16周龄ApoE-/-小鼠较8周龄ApoE-/-小鼠主动脉根部IMT增厚更为明显(P<0.01)。采用UBM所测IMT与病理测值呈高度正相关(r=0.81,P<0.0001)。结论 采用UBM成像可准确测量小鼠主动脉IMT,观测ApoE-/-小鼠升主动脉早期动脉粥样硬化的病变特征。 |
| 英文摘要: |
| Objective To analyze the ultrasonic characteristics of aortic atherosclerosis of Apolipoprotein-E knockout (ApoE-/-) mice in the early stage with ultrasound biomicroscopy (UBM). Methods Two different age groups of male ApoE-/- mice (8 and 16 weeks, each n=8) were used as experimental groups. Another two groups of age-matched male C57BL/6 mice (8 and 16 weeks, each n=8) were used as control groups. The morphosis of the aortic root, ascending aorta and aortic arch were observed with UBM, and the intima-media thickness (IMT) was measured at aortic root short axis view and compared with corresponding histological measurements in the same vascular section. Results UBM showed IMT was thicker in ApoE-/- mice than that of the controls at the same age (P<0.01). Blinded UBM-based and histological measurements of IMT showed significant age-dependant increase of IMT from 8 to 16 weeks in Apoe-/- mice (P<0.01). IMT measured with UBM was positively correlated with histological measurement at the same vascular region (r=0.81, P<0.0001). Conclusion Compared with histopathology, UBM can detect atherosclerotic progression in ApoE-/- mice through measuring IMT of the aorta, especially at the early stage of atherosclerosis. |
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