| 鄢雄,郭大静,余聪,王志刚,敖梦.聚乳酸-羟基乙酸共聚物包裹Gd-DTPA微球的弛豫性能和体外释放规律[J].中国医学影像技术,2012,28(1):35~39 |
| 聚乳酸-羟基乙酸共聚物包裹Gd-DTPA微球的弛豫性能和体外释放规律 |
| Relaxation properties and in vitro release law of Gd-DTPA-loaded polylactic acid glycolic acid microspheres |
| 投稿时间：2011-06-22 修订日期：2011-08-14 |
| DOI： |
| 中文关键词: 聚乳酸-羟基乙酸共聚物 钆DTPA 弛豫性能 磁共振成像 对比剂 |
| 英文关键词:Polylactic acid glycolic acid Gadolinium DTPA Relaxation properties Magnetic resonance imaging Contrast media |
| 基金项目:重庆市自然科学基金计划(CSTC,2008BB5233)。 |
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| 中文摘要: |
| 目的 制备聚乳酸-羟基乙酸共聚物(PLGA)包裹Gd-DTPA微球制成Gd-PLGA,观察其弛豫性能、体外释放规律,为构建靶向MR分子探针打下基础。方法 采用乳化溶剂蒸发法(水/油/水)制备Gd-PLGA,高效液相色谱法测定Gd-DTPA含量,以离心法测量Gd-PLGA微球包封率。采用MR扫描仪测定T1,计算Gd-PLGA微球的弛预率(R1);在磷酸盐缓冲液(PBS组)和双蒸水环境下(双蒸水组)模拟Gd-PLGA的体外释放规律。结果 成功制备出Gd-PLGA,被包裹的Gd-DTPA为15.00 mg,包封率为31.99%,包裹后R1弛豫性能下降(P=0.008)。体外释放规律研究发现微球在双蒸水环境释放量多于PBS,但组间差异无统计学意义(P=0.691),组间单位时间累计释放曲线形态相似。结论 采用乳化溶剂蒸发法成功制备出Gd-PLGA,Gd-DTPA被PLGA包裹后R1下降,释放规律与其他PLGA微球体系相似。 |
| 英文摘要: |
| Objective To prepare Gd-DTPA-loaded polylactic acid glycolic acid (PLGA) microspheres (Gd-PLGA) for MR contrast agents, and to research its relaxation properties, in vitro release, in order to lay foundation for MR molecular imaging probe. Methods Gd-PLGA was prepared by emulsion solvent evaporation method (water/oil/water). The content of Gd-DTPA was determined by using high performance liquid chromatography method, then the encapsulation efficiency of Gd-PLGA was measured by using centrifugation method. T1 relaxation times were measured by MR scanner, and then R1 relaxation properties of Gd-PLGA were calculated. The in vitro release laws of Gd-PLGA in environment of phosphate buffer solution (PBS) and double distilled water were simulated. Results Gd-PLGA was successfully prepared, the amount of encapsulated Gd-DTPA was 15.00 mg, and the encapsulation efficiency was 31.99%. The relaxation properties of Gd-DTPA decreased after wrapped with PLGA (P=0.008). The amount of release in the environment of double distilled water was more than that in the environment of PBS, but no statistical difference was observed (P=0.691). The cumulative release curves were similar between the environment of double distilled water and PBS. Conclusion Gd-PLGA is prepared by emulsion solvent evaporation method. The relaxation properties of Gd-DTPA decreased after wrapped with PLGA. Gd-PLGA has similar natures with other microspheres system. |
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