| 景香香,刘洁,杨炳昂,符少清,吴汤娜,王东林.超声介导击破微泡增强型绿色荧光蛋白转染正常大鼠关节滑膜组织[J].中国医学影像技术,2011,27(12):2407~2409 |
| 超声介导击破微泡增强型绿色荧光蛋白转染正常大鼠关节滑膜组织 |
| Transfection of enhanced green fluorescent protein into knee joint synovial membrane of normal rats by ultrasound-mediated microbubble destruction |
| 投稿时间：2011-04-02 修订日期：2011-05-03 |
| DOI： |
| 中文关键词: 超声检查,介入性 微泡 关节 滑膜 基因转移技术 |
| 英文关键词:Ultrasonography, interventional Microbubbles Joints Synovial membrane Gene transfer techniques |
| 基金项目:国家自然科学基金(30860270)、海南省自然科学基金(30854)。 |
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| 中文摘要: |
| 目的 探讨超声击破微泡介导增强型绿色荧光蛋白(EGFP)转染大鼠关节滑膜组织的可行性。 方法 将20只正常清洁级Wister大鼠分为4组,每组5只(10个膝关节)。单纯质粒组:10 μg EGFP注射入大鼠关节腔内;超声+质粒组:EGFP注射入大鼠关节腔内,超声持续照射10 min;微泡+质粒组:300 μl SonoVue与10 μg EGFP混合注射入大鼠关节腔内;超声+微泡+质粒组:将300 μl SonoVue与10 μg EGFP混合后注射入大鼠关节腔内,超声持续照射10 min。3天后取出大鼠膝关节滑膜组织,直接贴在载玻片上,在荧光显微镜480 nm波长激发状态下观察EGFP转染效果。 结果 单纯质粒组、超声+质粒组和微泡+质粒组的大鼠膝关节滑膜组织EGFP的荧光强度稍有增强;超声+微泡+质粒组EGFP的荧光强度明显增强。 结论 超声介导微泡可以实现EGFP质粒对正常大鼠关节滑膜组织的转染。 |
| 英文摘要: |
| Objective To explore the feasibility of enhanced green fluorescent protein (EGFP) transfection into normal rat's knee joint synovial membrane by ultrasound-mediated microbubble destruction. Methods Twenty rats were divided into 4 groups (each including 5 rats/10 knees), i.e. EGFP group (injecting 10 μg EGFP into rats joints), ultrasound+EGFP group (irradiating with ultrasound for 10 min after 10 μg EGFP into rats joints), microbubbles+EGFP group (injecting 300 μl SonoVue and 10 μg EGFP into rats joints) and ultrasound+microbubbles+EGFP group (irradiating with ultrasound for 10 min after 300 μl SonoVue and 10 μg EGFP into rats joints). Rats were sacrificed after 3 days and knee joints synovial membrane tissues were observed for EGFP protein expression on fluorescence microscopy. Results Light EGFP expression was seen in synovial membrane tissues of EGFP group, ultrasound+EGFP group and microbubbles+EGFP group, while strong EGFP expression was seen in synovial membrane tissues of ultrasound+microbubbles+EGFP group. Conclusion It is possible to deliver EGFP into rat's joint synovial membrane tissues by ultrasound-mediated microbubble destruction. |
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