| 李振洲,李泉水,郑敏娟,Shu-ping GE,Xiong-wen CHEN.超声生物显微镜斑点追踪成像评价正常小鼠左心室内、外膜心肌应变[J].中国医学影像技术,2011,27(7):1325~1329 |
| 超声生物显微镜斑点追踪成像评价正常小鼠左心室内、外膜心肌应变 |
| Assessment of endocardial and epicardial left ventricular strain in normal mouse models with ultrasound biomicroscopy and speckle tracking imaging |
| 投稿时间：2011-02-12 修订日期：2011-03-03 |
| DOI： |
| 中文关键词: 显微镜,声学的 斑点追踪 小鼠 应变 |
| 英文关键词:Microscopy, acoustic Speckle tracking Mice Strain |
| 基金项目:深圳市医学重点专科建设经费(2005CD6)。 |
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| 中文摘要: |
| 目的 应用超声生物显微镜(UBM)及斑点追踪(STI)技术探讨不同月龄正常小鼠左心室内膜及外膜心肌应变的特征。 方法 对48只不同月龄的健康成年雄性小鼠行常规超声心动图检查,采集胸骨旁左心室长轴、乳头肌水平左心室短轴二维图像。常规超声心动图测量舒张末期室间隔厚度(IVST)、左心室后壁厚度(LVPWT)、左心室舒张末期内径(LVEDD)、左心室收缩末期内径(LVESD)、左心室射血分数(LVEF)、左心室内径缩短率(LVFS)。脱机分析两个不同切面左心室内、外膜的纵向应变、圆周应变及径向应变的峰值应变(PK)、达峰时间(TPk)、室壁最大达峰延迟时间(MOWD)等指标。 结果 不同月龄间心率、IVST、LVPWT、LVEDD和LVESD的差异无统计学意义。与3月龄组比较,12月龄组LVEF、LVFS均减小(P＜0.05)。随月龄增加,小鼠左心室内膜下和外膜下心肌纵向、圆周及径向PK均呈逐渐减小的趋势:与3月龄组相比,12月龄组的测值均明显减小(P＜0.05);与6月龄组相比,12月龄组径向应变测值也明显减小(P＜0.05)。外膜下心肌的纵向及圆周应变均显著小于内膜下心肌(P＜0.01)。随月龄增加,左心室内膜下和外膜下心肌纵向、圆周及径向TPk、MOWD均呈逐渐增大趋势。与3、6月龄组相比,12月龄组的部分测值明显增大(P＜0.05)。同月龄正常小鼠内膜下及外膜下心肌纵向、圆周TPk及MOWD接近(P＞0.05)。 结论 UBM及STI技术能够无创、简便地全面评价正常小鼠左心室内、外膜下心肌的应变;所获不同月龄正常小鼠心脏结构及功能的正常测量值范围可为今后其他小鼠心脏模型的研究提供参考依据。 |
| 英文摘要: |
| Objective To analyze the global strain of endocardium and epicardium in left ventricular (LV) of normal mouse models in different months by using ultrasound biomicroscopy (UBM) and speckle tracking imaging (STI). Methods Forty-eight normal male mice with different months were selected. General echocardiography was applied by using UBM for all the mice. Two-dimensional images of parasternal LV long-axis view and short-axis at the midventricular level were acquired, and the measurements such as interventricular septum thickness (IVST), left ventricular post wall thickness (LVPWT), left ventricular end-diastolic dimension (LVEDD), left ventricular end-systolic dimension (LVESD), left ventricular ejection fraction (LVEF) and left ventricular fraction shortening (LVFS) were obtained. Endocardial and epicardial strain in longitudinal, circumferential and radial directions were analyzed by using analysis software. Measurements such as peak systolic strain (PK), time to peak (TPk) and maximal opposite wall delay (MOWD) were all obtained. Results There was no statistical difference on the heart rate, IVST, LVPWT, LVEDD and LVESD among mice of different months. LVEF and LVFS of 12 months group decreased compared with 3 months group (P＜0.05). LV endocardial and epicardial PK in longitudinal, circumferential and radial directions decreased gradually along with the increasing of age. Comparing with 3 months group, all the PK measurement values decreased significantly in 12 months group. And comparing with 6 months group, the radial PK of 12 months group decreased significantly. Comparing with the endocardial PK, epicardial longitudinal and circumferential PK values decreased significantly (P＜0.01). With the increasing of age, endocardial and epicardial TPk and MOWD increased gradually. Comparing with 3 and 6 months groups, some values of 12 months group increased significantly (P＜0.05). In the mice of the same age, there was no difference between endocardium and epicardium about TPk and MOWD in longitudinal and circumferential directions (P>0.05). Conclusion The endocardial and epicardial strain of LV in normal mice can be measured noninvasively and conveniently by using UBM and STI. The results of the research for normal mice model of different months can be used as normal references in the future. |
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