| 郭倩,姜立新,胡兵.不同辐照模式聚焦超声诱导人胰腺癌细胞凋亡的量效关系[J].中国医学影像技术,2011,27(3):479~482 |
| 不同辐照模式聚焦超声诱导人胰腺癌细胞凋亡的量效关系 |
| Dose-effect of human pancreatic carcinoma cell apoptosis induced by focused ultrasound with different radiation patterns |
| 投稿时间：2010-09-14 修订日期：2010-11-15 |
| DOI： |
| 中文关键词: 高强度聚焦超声消融 细胞凋亡 胰腺肿瘤 辐照模式 |
| 英文关键词:High-intensity focused ultrasound ablation Apoptosis Pancreatic neoplasms Radiation pattern |
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| 中文摘要: |
| 目的 探讨不同辐照模式聚焦超声诱导人胰腺癌细胞凋亡的最佳辐照方案。方法 对人胰腺癌细胞系PaTu 8988t细胞悬液进行聚焦超声辐照:第1组为空白对照组,第2、3组采用脉冲辐照模式,第4、5组采用连续辐照模式,各组辐照剂量通过改变辐照时间来改变。以流式细胞仪检测辐照后24 h肿瘤细胞凋亡率及细胞死亡率。结果 超声辐照后各组的细胞悬液最高温度分别为28.00℃、(42.20±2.17)℃、(50.80±0.84)℃、(55.80±2.17)℃、(65.20±3.11)℃,各组间差异均有统计学意义(P均<0.05)。各辐照组的细胞凋亡率为(0.56±0.15)%、(1.28±0.16)%、(1.84±0.29)%、(5.74±1.15)%、(2.00±0.84)%,细胞死亡率为(3.28±0.59)%、(4.60±0.47)%、(8.64±2.69)%、(41.12±15.91)%、(49.70±8.02)%,各组间差异均有统计学意义(P均<0.05)。结论 聚焦超声辐照剂量在接近热凝固温度阈值时,采用连续辐照模式可获得较高的细胞凋亡率及细胞死亡率。 |
| 英文摘要: |
| Objective To explore the optimal radiation pattern of focused ultrasound induced pancreatic carcionoma cell apoptosis. Methods The human pancreatic carcinoma cell line PaTu 8988t suspension was radiated by focused ultrasound using 5 dose groups with different radiation time parameters and patterns. Pulsed radiation pattern was used in groups 2 and 3, while continuous radiation pattern was used in group 4 and 5, group 1 was taken as the controls. The ratio of focused ultrasound induced cell apoptosis and cell death was tested with flow cytometer 24 h after ultrasound radiation. Results The maximum cell suspension temperature of 5 dose groups was 28.00℃, (42.20±2.17)℃, (50.80±0.84)℃, (55.80±2.17)℃ and (65.20±3.11)℃, respectively (all P＜0.05). The cell apoptosis rate was (0.56±0.15)%, (1.28±0.16)%, (1.84±0.29)%, (5.74±1.15)% and (2.00±0.84)%, respectively for 5 dose groups, and the cell death rate was (3.28±0.59)%, (4.60±0.47)%, (8.64±2.69)%, (41.12±15.91)% and (49.70±8.02)%, respectively (all P＜0.05). Conclusion Using threshold thermal exposures of focused ultrasound radiation with continuous radiation pattern is able to acquire high ratio of cell apoptosis. |
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