| 张松,邹利光,张冬,杨华,戚跃勇,梁开运.MRI观察金磁微粒结合抗podoplanin抗体体外标记淋巴管内皮细胞[J].中国医学影像技术,2011,27(2):237~241 |
| MRI观察金磁微粒结合抗podoplanin抗体体外标记淋巴管内皮细胞 |
| MRI observation on lymphatic endothelial cell labeled by GoldMag and anti-podoplanin antibody in vitro |
| 投稿时间：2010-10-05 修订日期：2010-11-14 |
| DOI： |
| 中文关键词: 磁共振成像 分子成像 分子探针 PDPN蛋白,人 内皮细胞 |
| 英文关键词:Magnetic resonance imaging Molecular imaging Molecular probes PDPN protein, human Endothelial cells |
| 基金项目:国家自然科学基金面上项目(30770609、81071197)。 |
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| 中文摘要: |
| 目的 制备淋巴管内皮细胞(LEC)靶向分子探针(GoldMag-pod),探讨GoldMag-pod对LEC的体外标记情况及其在MR检查中对信号强度的影响。方法 将金磁微粒(GoldMag)与抗podoplanin抗体通过"一步法"耦联,制备GoldMag-pod,以分光光度计检测GoldMag的抗体耦联效率,ELISA法检测抗体与GoldMag结合前、后的免疫活性,免疫荧光染色、普鲁士蓝染色检测GoldMag-pod对LEC的标记情况。用不同浓度GoldMag-pod标记LEC后,观察GRE T2*WI信号强度的改变。结果 GoldMag与抗podoplanin抗体通过"一步法"耦联成功,GoldMag的抗体耦联效率为52.94%,抗podoplanin抗体与GoldMag结合后其免疫活力降低为结合前的42.05%;GoldMag-pod标记后的LEC发出特异性绿色荧光, 标记阳性率随GoldMag-pod浓度的增高而增高。GoldMag-pod标记后的LEC悬液的GRE T2*WI信号强度明显降低。结论 GoldMag与抗podoplanin抗体耦联制备的分子探针GoldMag-pod具有较高免疫活性,能特异性标记LEC,产生MR靶向增强作用。 |
| 英文摘要: |
| Objective To synthesize molecular probe (GoldMag-pod) which can label lymphatic endothelial cell (LEC),and to investigate the labeling efficiency and MRI enhancement of the molecular probe in vitro. Methods GoldMag-pod was synthesized by using single-step coupling process of GoldMag and anti-podoplanin antibody. The coupling efficiency (CE) of GoldMag was determined with UV-Vis spectroscopy. ELISA was performed to evaluate the influence of GoldMag on antibody immunoactivity. The LEC label status of GoldMag-pod was observed with immunofluorescence and Prussian blue staining. After labeling by a range of different concentrations of GoldMag-pod, the variation of GRE T2*WI signal intensity of the solutions of LEC was investigated. Results GoldMag-pod was successfully synthesized, and the CE of GoldMag was 52.94%. The immunoactivity of GoldMag-pod was 42.05% of original antibody. LEC showed green fluorescence after immunofluorescence staining, and the results of Prussian blue staining showed that the positive label rate of LEC increased as the concentration of GoldMag-pod raised up. After labeled by GoldMag-pod, GRE T2*WI signal intensity of LEC solution decreased significantly. Conclusion The molecular probe GoldMag-pod coupled by GoldMag and anti-podoplanin antibody has relative high immunoactivity, which can specifically label LEC and result in targeted enhancement of MR imaging. |
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