| 李巧,冉海涛,王志刚,钟世根,凌智瑜,李攀,李兴升,陈庆伟.超声微泡联合Ad-EGFP/HIF-1α介导EPCs归巢大鼠缺血心肌[J].中国医学影像技术,2010,26(11):2030~2033 |
| 超声微泡联合Ad-EGFP/HIF-1α介导EPCs归巢大鼠缺血心肌 |
| Myocardium-targeted homing of intravenously implantated EPCs using ultrasound microbubbles and Ad-EGFP/HIF-1α |
| 投稿时间：2010-05-20 修订日期：2010-07-11 |
| DOI： |
| 中文关键词: 超声检查 造影剂 重组腺病毒 内皮祖细胞 心肌梗死 |
| 英文关键词:Ultrasonography Contrast media Recombinant adenoviruses Endothelial progenitor cells Myocardial infarction |
| 基金项目:国家自然科学基金面上项目(30970826)、国家自然科学基金青年科学基金项目(30800271)。 |
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| 中文摘要: |
| 目的 观察超声破坏微泡联合Ad-EGFP/HIF-1α介导EPCs移植归巢大鼠缺血心肌的可行性及有效性。方法 采用Percoll密度梯度离心法分离SD大鼠骨髓人脐血单个核细胞(MNCs),血管内皮生长因子(VEGF)、碱性成纤维细胞生长因子(bFGF)及表皮生长因子(EGF)诱导培养;Ad-EGFP/HIF-1α在HNK293细胞中进行扩增,之后感染体外培养的内皮祖细胞(EPCs)。将30只SD大鼠建立MI模型后随机分为5组:①空白对照组(C),②超声＋微泡组(US+MB),③单纯EPCs组(EPCs),④超声＋EPCs组(US+EPCs),⑤超声＋微泡+EPCs组(US+MB+EPCs)。EPCs移植后48 h处死大鼠,以激光共聚焦显微镜观察大鼠心肌组织内EPCs的分布,Western blot 检测HIF-1α的表达。结果 US+MB+EPCs组绿色荧光强度高于其他各组,Western blot显示,US+MB+EPCs组HIF-1α蛋白表达高于其他组(P<0.05)。结论 超声破坏微泡联合Ad-EGFP/HIF-1α介导EPCs移植可有效促进EPCs归巢,为干细胞移植治疗IHD提供了一种新的途径。 |
| 英文摘要: |
| Objective To explore the feasibility of myocardium-targeted homing of intravenously implantated EPCs using ultrasound microbubbles and Ad-EGFP/HIF-1α. Methods The mononu-clear cells were isolated from rat bone marrow with percoll density gradient centrifugation and induced with vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF). Ad-EGFP/HIF-1α was amplified in HNK293 cells, and then transfected EPCs in vitro. Thirty Sprague Dawley rats were randomly divided into 5 groups after the models of myocardial infarction being prepared: ①blank control group (C), ②ultrasound+microbubbles group (US+MB), ③endothelial progenitor cells group (EPCs), ④ultrasound+Endothelial progenitor cells group (US+EPCs), ⑤ultrasound+microbubbles+endothelial progenitor cells group (US+MB+EPCs). All the rats were sacrificed 48 h after EPCs implantation. The intensity of EGFP in the rat myocardium was observed with laser confocal microscopy. The expression of HIF-1α was respectively detected with Western blot. Results The EGFP intensity in US+MB+EPCs group was the highest among all 5 groups, and its expression of HIF-1α was higher than those of other groups. Conclusion Intravenously implantated EPCs using ultrasound microbubbles and Ad-EGFP/HIF-1α can augment the homing ability of EPCs to the infracted myocardium, which providing a novel strategy for EPCs implantation of ischemic heart disease. |
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