| 杨莉,刘政,左松,谭开彬,高云华,刘平,李秋颖.携带凝血酶原复合物的阴离子脂膜微泡的制备[J].中国医学影像技术,2010,26(11):2023~2026 |
| 携带凝血酶原复合物的阴离子脂膜微泡的制备 |
| Preparation of anionic lipid-coated microbubbles binding prothrombin complex concentrate |
| 投稿时间：2010-03-18 修订日期：2010-09-05 |
| DOI： |
| 中文关键词: 阴离子脂膜微泡 凝血酶原复合物 理化性质 |
| 英文关键词:Anionic lipid-coated microbubble Prothrombin complex concentrate Physiochemical properties |
| 基金项目:国家自然科学基金(30470468)。 |
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| 中文摘要: |
| 目的 探索一种携带凝血酶原复合物(PCC)的阴离子脂膜微泡的制备方法。方法 首先制备阴离子脂膜微泡并评价其理化性质;继而采用直接连接法(黏附法、整合法)和亲和素-生物素法分别使阴离子微泡携带PCC,观察并检测洗涤前、后微泡的理化性质、PCC与微泡的结合情况,并对所结合PCC中Ⅸ因子的凝血活性进行评价。结果 阴离子脂膜微泡稳定,微泡表面电位均值－62.70 mV;采用直接连接法(黏附法、整合法)制备的携带PCC的阴离子脂膜微泡洗涤前、后微泡与FITC-PCC的结合率均较高,且能在洗涤前保持较高活性,组间差异均无统计学意义(P均>0.05);亲和素-生物素法制备的携带PCC阴离子脂膜微泡,各种制备方法间比较,微泡与链酶亲和素的结合率均较高,洗涤前、后各组间差异均无统计学意义(P均>0.05),但生物素化后PCC中Ⅸ因子活性明显减低(降低约93.07%)。结论 直接连接法可成功制备携带PCC的阴离子脂膜微泡,且能保持较高的活性。直接连接法可使链酶亲和素与阴离子脂膜微泡很好地结合,但生物素化可能破坏PCC的活性。 |
| 英文摘要: |
| Objective To prepare anionic lipid-coated microbubbles (MB) binding prothrombin complex concentrate(PCC). Methods Anionic lipid-coated MB was prepared and its physiochemical properties were assessed. Then MB were bound with PCC through direct conjugation (before or after mechanical vibration) or Biotin-avidin bridge binding methods. The physiochemical properties of the MB were evaluated. Results MB were stable and the electronic potential was －62.70 mV. For direct binding, before or after repeated washing, the binding rate of MB and FITC-PCC in two different preparations were both high (P>0.05), and MB maintained the activities before washing. For Avidin-biotin Bridge binding methods, the binding rate of MB and streptoavidin was high and no difference was found between preparation methods (all P>0.05). The biotinylated process deactivated factor Ⅸ of PCC about 93.07%. Conclusion FITC-PCC concentrate that maintaining high activities can be conjugated with anionic lipid MB through direct binding during preparation procedure. Through direct binding, streptoavidin can be conjugated with anionic lipid microbubbles closely,whereas the biotinylated process of PCC can significantly deactivate the activity of factor Ⅸ. |
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