| 王秋实,刘辉,梁长虹,刘再毅,郑君惠,曾琼新,张明辉.磁共振氢质子波谱在兔肝纤维化模型中的应用[J].中国医学影像技术,2010,26(5):789~792 |
| 磁共振氢质子波谱在兔肝纤维化模型中的应用 |
| Application of proton magnetic resonance spectroscopy in the rabbit models of liver fibrosis |
| 投稿时间：2009-11-22 修订日期：2010-01-22 |
| DOI： |
| 中文关键词: 肝硬化 磁共振波谱 显微镜检查,电子 动物实验 |
| 英文关键词:Liver cirrhosis Magnetic resonance spectroscopy Microscopy, electron Animals, experimentation |
| 基金项目:广东省医学科研基金课题(A2009026)。 |
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| 中文摘要: |
| 目的 通过动物模型评价氢质子磁共振波谱(1H-MRS)在肝纤维化诊断中的应用价值。方法 对以四氯化碳诱导产生的肝纤维化模型兔和对照组兔行1H-MRS检查,获得肝脏主要含氢代谢物的波峰下面积,包括脂质(lipid)、胆碱(Cho)、糖原/葡萄糖复合物(Glyu),同时计算代谢物与脂质的波峰下面积比值(Cho/lipid、Glyu/lipid)。以病理学肝纤维化分期为基础将兔划分为无纤维化组(S0)、轻中度纤维化组(S1~S2)和重度纤维化/肝硬化组(S3~S4),比较不同组间1H-MRS参数变化情况,同时观察透射电镜下肝脏超微结构的变化。结果 Cho、Glyu和lipid在轻中度纤维化组降低,在重度纤维化/肝硬化组升高;Cho/lipid和Glyu/lipid随肝纤维化的加重而升高。重度纤维化/肝硬化组与另两组Cho/lipid比较,差异有统计学意义(P均<0.05),其余指标在纤维化各组间差异无统计学意义。肝组织超微结构改变在轻中度纤维化时以肝细胞损伤为主,在重度纤维化/肝硬化时肝细胞损伤与细胞外纤维基质沉积并重。结论 肝纤维化时肝细胞损伤引起代谢物减少,细胞外基质沉积阻碍代谢物排出,1H-MRS变化是对肝组织超微结构改变的综合反映。Cho/lipid是检测重度纤维化/肝硬化的有效指标。 |
| 英文摘要: |
| Objective To evaluate proton magnetic resonance spectroscopy (1H-MRS) in the diagnosis of liver fibrosis in rabbits. Methods Rabbits with liver fibrosis induced by carbon tetrachloride and controls were examined with 1H-MRS. According to histological fibrosis stage, the rabbits were divided into three groups: No fibrosis (S0), mild/moderate fibrosis (S1—S2), and severe fibrosis/cirrhosis (S3—S4). The peak areas of lipid, choline (Cho), glycogen and glucose complex (Glyu) as well as the ratios of Cho/lipid, Glyu/lipid were calculated and compared among groups of liver fibrosis. The ultrastructural changes of the liver were observed with transmission electronic microscope. Results Cho, Glyu, lipid decreased in mild/moderate fibrosis and increased in severe fibrosis/cirrhosis, while Cho/lipid and Glyu/lipid increased gradually as the severity of fibrosis progressed. Of all parameters, only Cho/lipid was statistically different between severe fibrosis/cirrhosis and the other groups of fibrosis (P<0.05). On electron microscopy, the main abnormalities in mild/moderate fibrosis were hepatocyte injury, while in severe fibrosis and cirrhosis, both hepatocyte injury and extracellular matrix deposition were prominent. Conclusion Destroyed hepatocytes result in reduction of intracellular metabolites, while excellular matrix accumulation disturbs excretion of metabolites. The spectral abnormalities of 1H-MRS in liver fibrosis are consistent with ultrastructural alterations. Cho/lipid is an effective marker for detecting severe fibrosis/cirrhosis. |
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