| 董虹美,王志刚,冉海涛,李攀,钟世根.低频超声辐照微泡造影剂增强米托蒽醌对乳腺癌细胞的毒性作用[J].中国医学影像技术,2010,26(3):401~404 |
| 低频超声辐照微泡造影剂增强米托蒽醌对乳腺癌细胞的毒性作用 |
| Enhancing cytotoxicity of Mitoxantrone on human breast cancer cell with microbubble contrast during low-frequency ultrasound exposure |
| 投稿时间：2009-08-18 修订日期：2009-10-13 |
| DOI： |
| 中文关键词: 低频超声 微泡 米托蒽醌 乳腺肿瘤 |
| 英文关键词:Low-frequency ultrasound Microbubble Mitoxantrone Breast neoplasms |
| 基金项目:国家自然科学基金面上项目(30770566、30770565)。 |
|
| 摘要点击次数: 2668 |
| 全文下载次数: 901 |
| 中文摘要: |
| 目的 观察超声辐照微泡造影剂对米托蒽醌杀伤人乳腺癌细胞MCF-7作用的影响,并探讨其作用机制。方法 以MTT法检测米托蒽醌对MCF-7细胞的细胞毒作用,计算其24 h的IC50值。将对数生长期的人乳腺癌细胞MCF-7分为单纯米托蒽醌组(D组)、超声+米托蒽醌组(U+D组)、超声+微泡+米托蒽醌组(U+M+D组)、空白对照组(C组)。以MTT法检测各组细胞活性, 高效液相色谱法测定用药各组细胞内米托蒽醌的含量,采用透射电镜观察MCF-7 细胞形态学特征。结果 米托蒽醌对MCF-7细胞的IC50值为2.87 μg/ml。各组细胞经处理后培养24 h,细胞活性率C组＞D组＞U+D组＞U+M+D组,各组间差异有统计学意义(P<0.05)。高效液相色谱法测定用药各组细胞内米托蒽醌的含量为U+M+D组＞U+D组＞D组,差异有统计学意义(P<0.05)。透射电镜可观察到MCF-7 细胞凋亡的典型形态学改变。结论 低频超声辐照可促进化疗药物进入肿瘤细胞内,增强化疗药物对肿瘤细胞的杀伤作用,而低频超声辐照微泡可进一步增强该效应。 |
| 英文摘要: |
| Objective To investigate the antitumor effect and its mechanism of microbubble contrast combined with Mitoxantrone exposed to low-frequency ultrasound on human breast cancer cell MCF-7. Methods MTT method was applied to examine the growth inhibition of MCF-7 treated with Mitoxantrone. MCF-7 cells were randomly divided into 4 groups: Mitoxantrone group (group D), ultrasound+Mitoxantrone group (group U+D), ultrasound+microbuble +Mitoxantrone group (group U+M+D) and control group (group C). The cytoactive of each group was examined with MTT. The intracellular drug content in each group was measured with high performance liquid chromatography. The morphology of MCF-7 cells apoptosis was observed with transmission electron microscopy (TEM). Results The IC50 of Mitoxantrone was 2.87 μg/ml. The differences of cytoactive among all groups were significant (P<0.05). The intracellular drug content of group U+M+D was higher than that of group U+D, and the latter was higher than that of group D. The morphological changes of apoptosis were observed with TEM. Conclusion Low-frequency ultrasound can promote intracellular drug content as to enhance the sensitivity of chemotherapy drugs on tumor cells, and this effect can be enhanced by microbubble contrast exposure to low-frequency ultrasound. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
