| 李红丽,杜联芳,郑孝志,王慧萍,顾青,陈霞芳.超声辐照微泡介导脂质体小干扰RNA转染视网膜色素上皮细胞[J].中国医学影像技术,2010,26(1):22~24 |
| 超声辐照微泡介导脂质体小干扰RNA转染视网膜色素上皮细胞 |
| Ultrasound-targeted microbubble mediated liposome small interference RNA transfection to retinal pigment epithelium cells |
| 投稿时间：2009-06-01 修订日期：2009-09-01 |
| DOI： |
| 中文关键词: 超声 微泡 小干扰RNA 视网膜色素上皮细胞 |
| 英文关键词:Ultrasound Microbubble Small interference RNA Retinal pigment epithelium cells |
| 基金项目:国家自然科学基金(30772369)。 |
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| 中文摘要: |
| 目的 探讨超声靶向破坏超声微泡介导脂质体小干扰RNA(siRNA)转染视网膜色素上皮(RPE)细胞的价值。 方法 将人及大鼠RPE细胞隔孔接种于24孔板中(2×105/孔、1×105/孔),分5组处理:siRNA＋超声(US)、siRNA＋微泡(MBs)＋US、siRNA＋脂质体(L)、siRNA＋L＋US、siRNA＋L＋MBs＋US。12小时后,荧光显微镜观察并应用流式细胞仪测定阳性细胞比例。 结果 在无脂质体的条件下,超声或超声联合微泡不能促进siRNA转染人及大鼠RPE细胞。siRNA＋L＋US组siRNA转染人RPE细胞效率最高。siRNA＋L＋US＋MB组siRNA转染人及大鼠RPE细胞的效率显著低于siRNA＋L＋US组。 结论 超声辐照可促进脂质体介导的siRNA转染人RPE细胞。 |
| 英文摘要: |
| Objective To investigate the transfection efficiency of combination of ultrasound (US) microbubbles (MBs) mediated liposome small interference RNA (siRNA) to human and rat retinal pigment epithelium (RPE) cells. Methods Human and rat RPE cells and siRNA were incubated in 24-well plates (2×105/well and 1×105/well, respectively). The cells were devided into 5 groups: siRNA＋US, siRNA＋MBs＋US, siRNA＋L (liposome), siRNA＋L＋US, siRNA＋L＋US＋MBs. After 12 h, transfection efficiency was observed with fluorescence microscopy and flow cytometry. Results US or ultrasound targeted microbubbles destruction without liposome-mediated could not promote siRNA transfection efficiency to human and rat RPE cells. The transfection efficiency of human and rat RPE cells significantly decreased in the siRNA＋L＋US＋MBs group, but increased in siRNA＋L＋US group. Conclusion Ultrasonic irradiation can promote lipid-mediated siRNA transfected human RPE cells. |
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