| 陈铟铟,郭亮,王希明,洪若瑜,李勇刚,朱银娣.采用自制超小超顺磁性氧化铁行兔VX2肿瘤MR成像[J].中国医学影像技术,2009,25(11):1945~1948 |
| 采用自制超小超顺磁性氧化铁行兔VX2肿瘤MR成像 |
| MR imaging of rabbit VX2 tumor with self-made ultrasmall superparamagnetic iron oxide |
| 投稿时间：2009-04-13 修订日期：2009-06-16 |
| DOI： |
| 中文关键词: 葡聚糖 超小超顺磁性氧化铁 肿瘤 磁共振成像 |
| 英文关键词:Dextran Ultrasmall superparamagnetic iron oxide Neoplasms Magnetic resonance imaging |
| 基金项目: |
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| 摘要点击次数: 2057 |
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| 中文摘要: |
| 目的 观察自制USPIO在兔VX2肿瘤MR成像中的价值。方法 以一步法实现葡聚糖对Fe3O4纳米粒子的表面包裹,制备USPIO。7只新西兰大白兔双侧大腿肌肉内接种VX2肿瘤;6只于接种后2周分别行Gd-DTPA和USPIO T2*WI增强扫描。1只未注射对比剂的实验兔留电镜和元素分析作对照研究。计算不同对比剂的病灶对比度噪声比(CNR)和增强对比度。MR成像结束后,行普鲁士蓝染色、电镜及元素分析。结果 经傅立叶变换红外光谱仪证实,葡聚糖成功包裹到Fe3O4磁性纳米粒子的表面;且合成USPIO大小在10~20 nm,分散性较好。增强扫描中两种对比剂的CNR差异有统计学意义(P<0.005)。病理、电镜及元素分析共同证实肿瘤组织与正常肌肉USPIO的含量有显著差异。结论 VX2肿瘤血管基底膜对USPIO具有高通透性。USPIO T2*WI增强扫描可以提高肿瘤组织的增强效果。自制USPIO可被肿瘤细胞吞噬,是用于肿瘤成像的理想对比剂。 |
| 英文摘要: |
| Objective To investigate the value of self-made USPIO in VX2 tumor MR imaging in rabbits. Methods USPIO was synthesized by single step in which Fe3O4 nanoparticles was packaged by dextran. Seven rabbits were inoculated VX2 carcinoma in both thigh, and 6 rabbits with VX2 carcinoma in both thighs underwent MR imaging with Gd-DTPA and USPIO after two weeks of inoculation. The remain one was used for control study in electron microscope and elemental analysis. Contrast-noise ratio (CNR) and contrast enhancement were assessed. After MR imaging, Prussian blue staining, transmission electron microscope and elemental analysis were done. Results The Fe3O4 nanoparticles was packaged by dextran successfully on the surface confirmed by Fourier transformation infared spectrometer; the diameter of USPIO was 10-20 nm. The CNR of tumor had statistical difference between two contrast medium (P<0.005). The results of pathology, transmission electron microscope and elemental analysis all confirmed that the content of USPIO had notable difference in tumor and muscle. Conclusion Microvascular hyperpermeability exists in rabbit VX2 tumor. USPIO T2*WI enhancement scanning can increase the enhancement effect of tumor. Self-made USPIO can be phagocytized by tumor cell, which is an ideal contrast agent for tumor imaging. |
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