| 宋英儒,叶伟,黄仲奎,滕夏虹,张愚,邹春林.食蟹猴糖尿病模型脑部MR检查的实验研究[J].中国医学影像技术,2009,25(10):1745~1748 |
| 食蟹猴糖尿病模型脑部MR检查的实验研究 |
| MR experiments study on the brain of cynomolgus of diabetic models |
| 投稿时间：2009-04-22 修订日期：2009-05-07 |
| DOI： |
| 中文关键词: 糖尿病 食蟹猴 磁共振波谱 |
| 英文关键词:Diabetes mellitus Macaca fascicularis Magnetic resonance spectroscopy |
| 基金项目:国家高技术研究发展计划(2006AA02A112)、国家重点基础研究发展计划(2007CB947704)。 |
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| 中文摘要: |
| 目的 利用MRI和磁共振氢质子波谱(1H-MRS)技术研究食蟹猴糖尿病模型不同脑区代谢物的变化,探讨其在糖尿病脑病早期诊断中的价值。方法 静脉注射100 mg/kg 体质量的链脲佐菌素(STZ),诱导5只青年食蟹猴制作成1型糖尿病模型,经长期血糖监测和静脉糖耐量实验来评价该模型的可靠性及稳定性;造模3年后,通过MRI和1H-MRS技术分别对糖尿病模型猴的海马、颞叶和枕叶的形态、大小、信号及其代谢物:N-乙酰天门冬氨酸(NAA)、胆碱(Cho)和肌醇(mI)与肌酸(Cr)的比值进行测定,并通过放免法检测血清皮质醇的含量。选取5只健康成年猴作为对照组,同时进行MRI和1H-MRS检测。结果 ①静脉注射100 mg/kg 体质量STZ可长期诱导稳定的食蟹猴1型糖尿病模型;②1型糖尿病3年的食蟹猴海马mI/Cr比值高于对照组(P<0.05);③糖尿病3年的食蟹猴血清皮质醇的含量与对照组比较差异无统计学意义(P＞0.05)。结论 脑1H-MRS分析可检测到糖尿病食蟹猴海马早期病变中一些代谢物含量的变化,有可能为今后临床早期诊断糖尿病脑病提供帮助。 |
| 英文摘要: |
| Objective To investigate the value of MRI and 1H-MRS in diagnosis of early stage of diabetic encephalopathy by detecting regional metabolite in cynomolgus diabetes models. Methods Five pathogen-free male adolescent cynomolgus were made type 1 diabetes mellitus models (T1DM) by intravenous injection of streptozotocin (STZ) (100 mg/kg), and the reliability and stability of the modes were assessed with long term follow-up of blood glucose and intravenous glucose tolerance tests. MRI and 1H-MRS were performed to evaluate the volume, signal intensity and metabolic ratios of NAA/Cr, mI/Cr and Cho/Cr at hippocampus, lateral temporal lobe and occipital lobe 3 years after model establishment. Cortisol in serum was detected with immunoradiometric assay. In addition, 5 normal adult cynomolgus monkeys were selected in the control group and accepted the same examination above. Results ①Intravenous administration of STZ could made stable T1DM monkey model. ②Only mI/Cr ratio increased at hippocampus of diabetic monkeys compared to the control group (P<0.05). ③There was no statistical difference of cortisol in serum between the diabetic group and the control group (P＞0.05). Conclusion 1H-MRS may detect the metabolic changes of the hippocampus in STZ-induced diabetic adolescent cynomolgus monkeys and may contributes to the early diagnosis of diabetic encephalopathy. |
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