| 徐清宇,张秀明,姚群立,滕皋军,张宇.大鼠缺血性脑卒中后炎症反应的USPIO增强7.0TMRI观察[J].中国医学影像技术,2009,25(7):1134~1137 |
| 大鼠缺血性脑卒中后炎症反应的USPIO增强7.0TMRI观察 |
| USPIO enhanced 7.0T MRI research of inflammation in rat ischemic stroke |
| 投稿时间：2009-01-09 修订日期：2009-04-03 |
| DOI： |
| 中文关键词: 超微超顺磁氧化铁颗粒 巨噬细胞 炎症 脑血管意外 |
| 英文关键词:Ultrasmall superparamagnetic particles of iron oxide Macrophage Inflammation Cerebrovascular accident |
| 基金项目: |
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| 中文摘要: |
| 目的 观察USPIO诱导的信号改变与脑组织切片含铁巨噬细胞的分布是否一致。方法 38只SD大鼠随机分成两组,假手术组大鼠3只,模型组35只,其中模型组按7.0T MR扫描时间点(6 h、12 h、24 h、48 h、72 h)平均分成5组。大鼠缺血性脑卒中模型建立成功后,经大鼠尾静脉注射USPIO(对照组仅注射等量的生理盐水)后,于不同时间点行MR扫描,结束后处死模型大鼠,取脑组织石蜡切片做HE染色观察细胞死亡,普鲁士蓝染色观察铁颗粒,CD68免疫组织化学染色观察活化巨噬细胞。结果 模型组病灶T2WI高信号水肿区在注射USPIO后可见到负性增强,信号强度改变以48~72 h较明显,两者组间差异无统计学意义;而相应的T1WI信号呈正性增强,信号强度改变也是以48~72 h较明显。普鲁士蓝染色证实病灶周边可见铁粒子沉积,CD68免疫组化显示脑内小胶质细胞增生活跃,视野小胶质细胞计数以72 h最明显。结论 USPIO可以作为活体监测大鼠缺血性脑卒中后炎症反应的对比剂,其诱导的信号改变与脑组织切片含铁巨噬细胞的分布基本一致,内吞USPIO的细胞主要是激活的巨噬细胞。 |
| 英文摘要: |
| Objective To observe signal changes induced by USPIO accords with iron swallowed by macrophages in brain tissue sections in rats. Methods Thirty-eight SD rats were divided into two groups randomly. Three of them were involved in sham operation group, other thirty-five rats were divided into five subgroups averagely according to 7.0T MR scanning time (6 h, 12 h, 24 h, 48 h and 72 h). After establishment of MCAO models, USPIO was injected to tail intravenous and monitored with high resolution MRI at different time point, while rats in control group were injected with the same dose of sodium chloride. Brain tissue wax section was acquired after MR scanning. Cell necrosis, iron particle and activated macrophages were observed with HE dying, Prussian Blue dying and CD68 immunochemistry staining respectively. Results The ischemic lesion was detected as hyperintense area on T2WI after occlusion and perfusion of MCA. The accumulation of USPIO appeared as hypointense on T2WI but hyperintense on T1WI. The maximum signal change was observed at 48-72 h in both T1WI and T2WI (P>0.05). The iron particle accumulation was found in the boundary of ischemic lesion and necrotic area with Prussian Blue dying. Activated microglia was manifested with CD68 immunochemistry staining, the number of microglia at 72 h was more than those of the other time points. Conclusion USPIO can be used as a contrast agent to monitor rat ischemic stroke in vivo, and the signal changes induced by USPIO approximately accord with iron swallowed by macrophages in brain tissue sections. The cells which swallow USPIO are mainly activated macrophages. |
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